Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Culture of primary epithelial adenoma cells from familial adenomatous polyposis patients
Δημιουργός/Συγγραφέας:	Fostira, Florentia Apessos, Angela Oikonomou, Eutyxia Kouklis, Panos Baratsis, Sotiris Manifikos, Grigoris Andera, Ladislav Yannoukakos, Drakoulis [EL] Πίντζας, Αλέξανδρος[EN] Pintzas, Alexander Nasioulas, George
Εκδότης:	International Institute of Anticancer Research
Τόπος έκδοσης:	ATHENS
Ημερομηνία:	2008-03-04
Γλώσσα:	Αγγλικά
ISSN:	0250-7005
Περίληψη:	Background: Colorectal tumors arise from unregulated cell proliferation of the intestinal epithelium through a multistep process the first step usually being premalignant adenomas. Familial adenomatous polyposis patients carry a germ line mutation in the APC gene leading to the development of thousands of polyps, which, if left untreated, lead to cancer. The goal of this study was the establishment of conditions for the culture of epithelial cells composing an adenomatic structure. Materials and Methods: All colorectal specimens were obtained from FAP patients within 1-2 hours of surgery. Cells were cultured by standard procedures. Characterization was carried out by immunostaining with pancytokeratin, vimentin and desmoplakin antibodies. Results: A culture protocol that gave rise to epithelial cell growth with high efficiency and efficacy was established. Successful subculturing of the cell sheets took place only when dispase prepared in Ca2+ and Mg2+ free medium, was used to digest polyp tissue taken from FAP patients. By using immunostaining these cells were characterized as epithelial. Conclusion: The protocol we developed here provides a means of preparing cell cultures from human colorectal adenomas, which aid in the research of the transition from adenoma to carcinoma.
Τίτλος πηγής δημοσίευσης:	Anticancer Research
Τόμος/Κεφάλαιο:	28
Τεύχος:	2A
Σελίδες:	843-846
Θεματική Κατηγορία:	[EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens)
Λέξεις-Κλειδιά:	polyp adenoma primary culture carcinogenesis cell culture
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© INT INST ANTICANCER RESEARCH
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο