Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin
Δημιουργός/Συγγραφέας:	Markopoulou, Soultana Kontargiris, Evangelos Batsi, Christina Tzavaras, Theodore [EL] Τρουγκάκος, Ιωάννης Π.[EN] Trougakos, Ioannis P. Boothman, David A. [EL] Γκόνος, Ευστάθιος[EN] Gonos, Efstathios S. Kolettas, Evangelos
Εκδότης:	Wiley-Blackwell Publishing Ltd.
Τόπος έκδοσης:	MALDEN
Ημερομηνία:	2009-07
Γλώσσα:	Αγγλικά
ISSN:	1742-464X
DOI:	10.1111/j.1742-4658.2009.07093.x
Περίληψη:	Vanadium exerts a variety of biological effects, including antiproliferative responses through activation of the respective signaling pathways and the generation of reactive oxygen species. As epidermal cells are exposed to environmental insults, human keratinocytes (HaCaT) were used to investigate the mechanism of the antiproliferative effects of vanadyl(IV) sulfate (VOSO(4)). Treatment of HaCaT cells with VOSO(4) inhibited proliferation and induced apoptosis in a dose-dependent manner. Inhibition of proliferation was associated with downregulation of cyclins D1 and E, E2F1, and the cyclin-dependent kinase inhibitors p21(Cip1/Waf1) and p27(Kip1). Induction of apoptosis correlated with upregulation of the c-fos oncoprotein, changes in the expression of clusterin (CLU), an altered ratio of antiapoptotic to proapoptotic Bcl-2 protein family members, and poly(ADP-ribose) polymerase-1 cleavage. Forced overexpression of c-fos induced apoptosis in HaCaT cells that correlated with secretory CLU downregulation and upregulation of nuclear CLU (nCLU), a pro-death protein. Overexpression of Bcl-2 protected HaCaT cells from vanadium-induced apoptosis, whereas secretory CLU overexpression offered no cytoprotection. In contrast, nCLU sensitized HaCaT cells to apoptosis. Our data suggest that vanadium-mediated apoptosis was promoted by c-fos, leading to alterations in CLU isoform processing and induction of the pro-death nCLU protein.
Τίτλος πηγής δημοσίευσης:	Febs Journal
Τόμος/Κεφάλαιο:	276
Τεύχος:	14
Σελίδες:	4208-4223
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	apoptosis Bcl-2 c-fos clusterin (CLU) vanadyl(IV) sulfate
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Όροι και προϋποθέσεις δικαιωμάτων:	Free access by the publisher. This article can be found at the following on Wiley Online Library: http://dx.doi.org/10.1111/j.1742-4658.2009.07093.x
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.blackwellpublishing.com/journals/EJB
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο