Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	hCINAP is an atypical mammalian nuclear adenylate kinase with an ATPase motif: Structural and functional studies
Δημιουργός/Συγγραφέας:	Drakou, Christina E. Malekkou, Anna Hayes, Joseph M. Lederer, Carsten W. [EL] Λεωνίδας, Δημήτρης Δ.[EN] Leonidas, Demetres D. [EL] Οικονομάκος, Νίκος Γ.[EN] Oikonomakos, Nikos G. Lamond, Angus I. Santama, Niovi [EL] Ζωγράφος, Σπύρος Ε.[EN] Zographos, Spyros E.
Εκδότης:	John Wiley & Sons, Inc.
Τόπος έκδοσης:	MALDEN
Ημερομηνία:	2012-01
Γλώσσα:	Αγγλικά
ISSN:	0887-3585
DOI:	10.1002/prot.23186
Περίληψη:	Human coilin interacting nuclear ATPase protein (hCINAP) directly interacts with coilin, a marker protein of Cajal Bodies (CBs), nuclear organelles involved in the maturation of small nuclear ribonucleoproteins UsnRNPs and snoRNPs. hCINAP has previously been designated as an adenylate kinase (AK6), but is very atypical as it exhibits unusually broad substrate specificity, structural features characteristic of ATPase/GTPase proteins (Walker motifs A and B) and also intrinsic ATPase activity. Despite its intriguing structure, unique properties and cellular localization, the enzymatic mechanism and biological function of hCINAP have remained poorly characterized. Here, we offer the first high-resolution structure of hCINAP in complex with the substrate ADP (and dADP), the structure of hCINAP with a sulfate ion bound at the AMP binding site, and the structure of the ternary complex hCINAP-Mg2+ADP-Pi. Induced fit docking calculations are used to predict the structure of the hCINAP-Mg2+ATP-AMP ternary complex. Structural analysis suggested a functional role for His79 in the Walker B motif. Kinetic analysis of mutant hCINAP-H79G indicates that His79 affects both AK and ATPase catalytic efficiency and induces homodimer formation. Finally, we show that in vivo expression of hCINAP-H79G in human cells is toxic and drastically deregulates the number and appearance of CBs in the cell nucleus. Our findings suggest that hCINAP may not simply regulate nucleotide homeostasis, but may have broader functionality, including control of CB assembly and disassembly in the nucleus of human cells. Proteins 2012;.
Τίτλος πηγής δημοσίευσης:	Proteins-structure Function and Bioinformatics
Τόμος/Κεφάλαιο:	80
Τεύχος:	1
Σελίδες:	206-220
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Φυσική[EN] Physics
Λέξεις-Κλειδιά:	crystal structure adenylate kinase 6 ATPase coilin Cajal bodies Biophysics
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
EU Grant identifier:	info:eu-repo/grantAgreement/EC/FP7/245866 info:eu-repo/grantAgreement/EC/FP7/230146
Κάτοχος πνευματικών δικαιωμάτων:	© 2011 Wiley Periodicals, Inc.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/cgi-bin/jhome/36176
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Οργανικής και Φαρμακευτικής Χημείας (ΙΟΦΧ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο