Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	DNA encapsulation via nanotemplates from cationic block copolymer micelles
Δημιουργός/Συγγραφέας:	Haladjova, E. Rangelov, S. Tsvetanov, Ch. B. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Εκδότης:	Royal Society Chemistry
Τόπος έκδοσης:	Cambridge
Ημερομηνία:	2012
Γλώσσα:	Αγγλικά
ISSN:	1744-683X
DOI:	10.1039/c2sm07029d
Περίληψη:	We report on a method for the encapsulation of DNA into polymeric nanocapsules. The encapsulation procedure involves three steps: formation of polyplexes between DNA and cationic polymeric micelles; coating of polyplexes with a cross-linked shell; dissociation of polyplexes. In the first step copolymer micelles were obtained by self-assembly of an amphiphilic polystyrene-b-poly(quaternized 2-vinylpyridine) (PS-b-PQ2VP) block copolymer in aqueous solution. Nanosized polyplexes (D-h = 190 nm and zeta = -21 mV) were formed upon the addition of DNA to the micellar solution at a phosphate/amine group ratio of 3 : 1. In the second step the complexes were coated with a crosslinked shell formed by seeded radical polymerization of N-isopropylacrylamide which resulted in a slight shift of zeta potential of the particles to a less negative value. The dissociation of polyplexes and removal of PS-b-PQ2VP were achieved by addition of salt and solvent exchange. Following rehydration via dialysis against water, polymeric nanocapsules with entrapped DNA were obtained. The nanocapsules were visualized by transmission electron microscopy; they exhibited smaller dimensions compared to the initial polyplexes and zeta potential very close to that of the pristine DNA.
Τίτλος πηγής δημοσίευσης:	Soft Matter
Τόμος/Κεφάλαιο:	8
Τεύχος:	10
Σελίδες:	2884-2889
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Επιστήμη (Γενικά)[EN] Science (General) [EL] Τεχνολογία (Γενικά)[EN] Technology (General) [EL] Φυσική[EN] Physics
Λέξεις-Κλειδιά:	Chemistry, Physical Materials Science, Multidisciplinary Physics, Multidisciplinary Polymer Science
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© ROYAL SOC CHEMISTRY
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο