Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Enhanced gene expression promoted by hybrid magnetic/cationic block copolymer micelles
Δημιουργός/Συγγραφέας:	Haladjova E. Rangelov S. Tsvetanov C.B. Posheva V. Peycheva E. Maximova V. Momekova D. Mountrichas G. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Bakandritsos A.
Εκδότης:	American Chemical Society
Ημερομηνία:	2014
Γλώσσα:	Αγγλικά
ISSN:	0743-7463
DOI:	10.1021/la501402q
Άλλο:	PubMed ID: 24945823
Περίληψη:	We report on novel gene delivery vector systems based on hybrid polymer-magnetic micelles. The hybrid micelles were prepared by codissolution of hydrophobically surface modified iron oxide and amphiphilic polystyrene-b-poly(quaternized 2-vinylpyridine) block copolymer (PS-b-P2QVP) in organic solvent. After extensive dialysis against water, micelles with positively charged hydrophilic corona of PQVP and hydrophobic PS core were prepared, in which magnetic nanoparticles were randomly distributed. The hybrid micelles were used to form complexes with linear (salmon sperm, 2000 bp, corresponding to Mw of 1.32 × 106 Da) and plasmid (pEGFP-N1, 4730 bp, corresponding to Mw of 3.12 × 10 6 Da) DNA. The resulting magnetopolyplexes of phosphate:amine (P/N) ratios in the 0.05-20 range were characterized by light scattering, ζ-potential measurements, and transmission electron microscopy as well as cytotoxicity and gel retardation assays. The investigated systems displayed a narrow size distribution, particle dimensions below 360 nm, whereas their ζ-potential values varied from positive to negative depending of the P/N ratio. The resulting vector nanosystems exhibited low toxicity. They were able to introduce pEGFP-N1 molecules into the cells. The application of a magnetic field markedly boosted the transgene expression efficiency of the magnetopolyplexes, which was even superior to those of commercial transfectants such as Lipofectamine and dendritic polyethylenimine.
Τίτλος πηγής δημοσίευσης:	Langmuir
Τόμος/Κεφάλαιο:	30
Τεύχος:	27
Σελίδες:	8193-8200
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2014 American Chemical Society.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο