Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Hydrophilic random cationic copolymers as polyplex-formation vectors for DNA
Δημιουργός/Συγγραφέας:	Chrysostomou, Varvara Katifelis, Hector Gazouli, Maria Dimas, Konstantinos Demetzos, Costas [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Εκδότης:	MDPI
Ημερομηνία:	2022
Γλώσσα:	Αγγλικά
DOI:	10.3390/ma15072650
Άλλο:	2650
Περίληψη:	Research on the improvement and fabrication of polymeric systems as non-viral gene delivery carriers is required for their implementation in gene therapy. Random copolymers have not been extensively utilized for these purposes. In this regard, double hydrophilic poly[(2-(dimethylamino) ethyl methacrylate)-co-(oligo(ethylene glycol) methyl ether methacrylate] [P(DMAEMA-co-OEGMA)] random copolymers were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The copolymers were further modified by quaternization of DMAEMA tertiary amine, producing the cationic P(QDMAEMA-co-OEGMA) derivatives. Fluorescence and ultraviolet–visible (UV–vis) spectroscopy revealed the efficient interaction of copolymers aggregates with linear DNAs of different lengths, forming polyplexes, with the quaternized copolymer aggregates exhibiting stronger binding affinity. Light scattering techniques evidenced the formation of polyplexes whose size, molar mass, and surface charge strongly depend on the N/P ratio (nitrogen (N) of the amine group of DMAEMA/QDMAEMA over phosphate (P) groups of DNA), DNA length, and length of the OEGMA chain. Polyplexes presented colloidal stability under physiological ionic strength as shown by dynamic light scattering. In vitro cytotoxicity of the empty nanocarriers was evaluated on HEK293 as a control cell line. P(DMAEMA-co-OEGMA) copolymer aggregates were further assessed for their biocompatibility on 4T1, MDA-MB-231, MCF-7, and T47D breast cancer cell lines presenting high cell viability rates.
Τίτλος πηγής δημοσίευσης:	Materials
Τόμος/Κεφάλαιο:	15
Τεύχος:	7
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	Non-viral vectors Gene delivery Nucleic acids DNA Random copolymers Polyplexes Cationic polymers RAFT polymerization In vitro cytotoxicity
Κάτοχος πνευματικών δικαιωμάτων:	2022 by the authors. Licensee MDPI, Basel, Switzerland.
Ηλεκτρονική διεύθυνση με ανοικτή πρόσβαση (link):	https://www.mdpi.com/1996-1944/15/7/2650
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο