Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	cPHB distribution and synthesis regulation by various induction forms of the AtoS–AtoC/Az in E. coli
Δημιουργός/Συγγραφέας:	Theodorou, E. C. Theodorou, M C Samali, M. N. Panagiotidis, C. A.
Εκδότης:	Wiley-Blackwell Publishing Ltd.
Ημερομηνία:	2007
Γλώσσα:	Αγγλικά
ISSN:	1742-464X
DOI:	10.1111/j.0014-2956.2007.05861_3.x
Περίληψη:	The AtoS–AtoC/Az two-component system and its target atoDAEB operon regulate poly-(R)-3-hydroxybutyrate (cPHB) biosynthesis upon acetoacetate- or spermidine-mediated induction. The response regulator AtoC/Az, the product of atoC gene, is the Az of ornithine decarboxylase. It belongs to the NtrC-NifA family of sigma54-RNA polymerase transcriptional activators. Many physiological roles have been proposed for Escherichia coli cPHB. We report, that a receiver domain-truncated form of AtoC/Az enhanced cPHB synthesis compared to wild type AtoC/Az overproducing cells. The constitutively active AtoC (Con), carrying mutation in C-terminal domain, resulted in basal cPHB levels. E. coli fadR mutants rendering the b-oxidation pathway constitutively active synthesized elevated cPHB compared to the basal cPHB levels in atoC (Con) fadR strains. A signal-dependent alteration of cPHB distribution was generated when the intracellular levels of the AtoS–AtoC/Az were increased. Specifically, acetoacetate enhanced cPHB accumulation initially in the membranes followed by an enhancement of cytoplasmic cPHB. Spermidine caused cPHB accumulation mainly in the membranes, suggesting interaction networks through AtoS–AtoC/Az system towards the cPHB regulation.
Τίτλος πηγής δημοσίευσης:	The FEBS Journal
Τόμος/Κεφάλαιο:	274
Τεύχος:	Suppl 1
Σελίδες:	196-196
Κάτοχος πνευματικών δικαιωμάτων:	© 2007 The Authors Journal compilation © 2007 FEBS
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/journal/119877016/tocgroup
Σημειώσεις:	Abstract
Εμφανίζεται στις συλλογές:	Άλλες δράσεις