@article{Calogeropoulou_Avlonitis_Minas_Alexi_Pantzou_Charalampopoulos_Zervou_Vergou_Katsanou_Lazaridis_et al._2009, title={Novel Dehydroepiandrosterone Derivatives with Antiapoptotic, Neuroprotective Activity}, volume={52}, ISSN={0022-2623}, archiveLocation={Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο}, url={https://hdl.handle.net/10442/12230}, DOI={10.1021/jm900468p}, abstractNote={DHEA analogues with modifications at positions C3 or 07 were synthesized and evaluated for neuroprotective activity against the neural-crest-derived PC12 cell model of serum deprivation-induced apoptosis. The most potent compounds were the spiro-epoxy derivatives 17 beta-spiro[5-androstene-17, 2’-oxiran]-3 beta-ol (20), (20S)-3 beta,21-dihydroxy-17 beta,20-epoxy-5-pregnene (23), and (20R)-3 beta,21-dihydroxy-17 alpha,20-epoxy-5-pregnene (27) with IG(50) values of 0.19 +/- 0.01, 99.0 +/- 4.6, and 6.4 +/- 0.3 nM, respectively. Analogues 20, 23, and 27, up to the micromolar range of concentrations, were unable to activate estrogen receptor alpha and beta (ER alpha and ER beta) or to interfere with ER-dependent gene expression significantly. In addition, they were unable to stimulate the growth of Ishikawa, MCF-7, and LNCaP cells. Our results suggest that the spiro-epoxyneurosteroid derivatives 20, 23, and 27 may prove to be lead molecules for the synthesis of novel neuroprotective agents.}, number={21}, journal={Journal of Medicinal Chemistry}, publisher={American Chemical Society}, author={Calogeropoulou, Theodora and Avlonitis, Nicolaos and Minas, Vassilios and Alexi, Xanthippi and Pantzou, Athanasia and Charalampopoulos, Ioannis and Zervou, Maria and Vergou, Varvara and Katsanou, Efrosini S. and Lazaridis, Iakovos and et al.}, year={2009}, month={Nov}, pages={6569–6587} }