@article{Papagiannopoulos A._Christoulaki A._Spiliopoulos N._Vradis A._Toprakcioglu C._ Pispas S._2015, title={Complexation of lysozyme with adsorbed PtBS-b-SCPI block polyelectrolyte micelles on silver surface}, volume={31}, ISSN={0743-7463}, archiveLocation={Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο}, url={https://hdl.handle.net/10442/17104}, DOI={10.1021/la504873h}, abstractNote={We present a study of the interaction of the positively charged model protein lysozyme with the negatively charged amphiphilic diblock polyelectrolyte micelles of poly(tert-butylstyrene-b-sodium (sulfamate/carboxylate)isoprene) (PtBS-b-SCPI) on the silver/water interface. The adsorption kinetics are monitored by surface plasmon resonance, and the surface morphology is probed by atomic force microscopy. The micellar adsorption is described by stretched-exponential kinetics, and the micellar layer morphology shows that the micelles do not lose their integrity upon adsorption. The complexation of lysozyme with the adsorbed micellar layers depends on the micelles arrangement and density in the underlying layer, and lysozyme follows the local morphology of the underlying roughness. When the micellar adsorbed amount is small, the layers show low capacity in protein complexation and low resistance in loading. When the micellar adsorbed amount is high, the situation is reversed. The adsorbed layers both with or without added protein are found to be irreversibly adsorbed on the Ag surface.}, number={2}, journal={Langmuir}, publisher={American Chemical Society}, author={Papagiannopoulos A. and Christoulaki A. and Spiliopoulos N. and Vradis A. and Toprakcioglu C. and Pispas S.}, year={2015}, pages={685–694} }