@article{Khemakhem M._ Papadimitriou V._ Sotiroudis G._ Zoumpoulakis P._ Arbez-Gindre C._Bouzouita N._ Sotiroudis T.G._2016, title={Melanin and humic acid-like polymer complex from olive mill waste waters. Part I. Isolation and characterization}, volume={203}, ISSN={0308-8146}, archiveLocation={Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο}, url={https://hdl.handle.net/10442/17473}, DOI={10.1016/j.foodchem.2016.01.110}, abstractNote={A water soluble humic acid and melanin-like polymer complex (OMWW-ASP) was isolated from olive mill waste waters (OMWW) by ammonium sulfate fractionation to be used as natural additive in food preparations. The dark polymer complex was further characterized by a variety of biochemical, physicochemical and spectroscopic techniques. OMWW-ASP is composed mainly of proteins associated with polyphenols and carbohydrates and the distribution of its relative molecular size was determined between about 5 and 190 kDa. SDS-PAGE shows the presence of a well separated protein band of 21.3 kDa and a low molecular weight peptide. The OMWW-ASP complex exhibits a monotonically increasing UV-Vis absorption spectrum and it contains stable radicals. Antioxidant activity measurements reveal the ability of the OMWW protein fraction to scavenge both the cationic 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical, as well as the stable nitroxide free radical 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL).}, journal={Food Chemistry}, publisher={Elsevier Ltd}, author={Khemakhem M. and Papadimitriou V. and Sotiroudis G. and Zoumpoulakis P. and Arbez-Gindre C. and Bouzouita N. and Sotiroudis T.G.}, year={2016}, pages={540–547} }