%0 Journal Article %A Khemakhem M.%A %A Papadimitriou V.%A %A Sotiroudis G.%A %A Zoumpoulakis P.%A %A Arbez-Gindre C.%A %A Bouzouita N.%A %A Sotiroudis T.G. %D 2016 %T Melanin and humic acid-like polymer complex from olive mill waste waters. Part I. Isolation and characterization %J Food Chemistry %V 203 %@ 0308-8146 %R 10.1016/j.foodchem.2016.01.110 %I Elsevier Ltd %P 540–547 %U https://hdl.handle.net/10442/17473 %X A water soluble humic acid and melanin-like polymer complex (OMWW-ASP) was isolated from olive mill waste waters (OMWW) by ammonium sulfate fractionation to be used as natural additive in food preparations. The dark polymer complex was further characterized by a variety of biochemical, physicochemical and spectroscopic techniques. OMWW-ASP is composed mainly of proteins associated with polyphenols and carbohydrates and the distribution of its relative molecular size was determined between about 5 and 190 kDa. SDS-PAGE shows the presence of a well separated protein band of 21.3 kDa and a low molecular weight peptide. The OMWW-ASP complex exhibits a monotonically increasing UV-Vis absorption spectrum and it contains stable radicals. Antioxidant activity measurements reveal the ability of the OMWW protein fraction to scavenge both the cationic 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical, as well as the stable nitroxide free radical 4-hydroxy-2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPOL). %> Αποθετήριο Ήλιος / ΕΙΕ