Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Performance in omics analyses of blood samples in long-term storage: Opportunities for the exploitation of existing biobanks in environmental health research
Δημιουργός/Συγγραφέας:	Hebels D.G.A.J. [EL] Γεωργιάδης, Παναγιώτης[EN] Georgiadis, Panagiotis Keun H.C. Athersuch T.J. Vineis P. Vermeulen R. Portengen U. Bergdahl I.A. Hallmans G. Palli D. Bendinelli B. Krogh V. Tumino R. Sacerdote C. Panico S. Kleinjans J.C.S. de Kok T.M.C.M. Smith M.T. [EL] Κυρτόπουλος, Σωτήριος Α.[EN] Kyrtopoulos, Soterios A.
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	0091-6765
DOI:	10.1289/ehp.1205657
Άλλο:	PubMed ID: 23384616
Περίληψη:	The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown. Objectives: We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles. Methods: We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at-80°C or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks. Results: Microarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ≥ 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of tim-in-storage was observed in samples stored over a period of 13-17 years. Conclusions: Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.
Τίτλος πηγής δημοσίευσης:	Environmental Health Perspectives
Τόμος/Κεφάλαιο:	121
Τεύχος:	4
Σελίδες:	480-487
Θεματική Κατηγορία:	[EL] Περιβαλλοντική υγεία[EN] Environmental health
Λέξεις-Κλειδιά:	Biomarkers Epigenomics Metabolomics Metabonomics Molecular epidemiology Proteomics Transcriptomics
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Bronze, Green
Σημειώσεις:	National Institute of Environmental Health Sciences, NIEHS: P42ES004705; Seventh Framework Programme, FP7: 226756; Medical Research Council, MRC: G0801056
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο