Παρακαλώ χρησιμοποιήστε αυτό το αναγνωριστικό για να παραπέμψετε ή να δημιουργήσετε σύνδεσμο προς αυτό το τεκμήριο:
https://hdl.handle.net/10442/17943
Εξειδίκευση τύπου : | Άρθρο σε επιστημονικό περιοδικό |
Τίτλος: | Bioenergetic profiling of the differentiating human MDS myeloid lineage with low and high bone marrow blast counts |
Δημιουργός/Συγγραφέας: | Poulaki, Aikaterini [EL] Κατσίλα, Θεοδώρα[EN] Katsila, Theodora Stergiou, Ioanna E Giannouli, Stavroula Gόmez-Tamayo, Jose Carlos Piperaki, Evangelia-Theophano Kambas, Konstantinos Dimitrakopoulou, Aglaia Patrinos, George P Tzioufas, Athanasios G Voulgarelis, Michael |
Ημερομηνία: | 2020-11-26 |
Γλώσσα: | Αγγλικά |
ISSN: | 2072-6694 |
DOI: | 10.3390/cancers12123520 |
Άλλο: | 33255926 |
Περίληψη: | Myelodysplastic syndromes (MDS) encompass a very heterogeneous group of clonal hematopoietic stem cell differentiation disorders with malignant potential and an elusive pathobiology. Given the central role of metabolism in effective differentiation, we performed an untargeted metabolomic analysis of differentiating myeloid lineage cells from MDS bone marrow aspirates that exhibited <5% (G1) or ≥5% (G2) blasts, in order to delineate its role in MDS severity and malignant potential. Bone marrow aspirates were collected from 14 previously untreated MDS patients (G1, n = 10 and G2, n = 4) and age matched controls (n = 5). Following myeloid lineage cell isolation, untargeted mass spectrometry-based metabolomics analysis was performed. Data were processed and analyzed using Metabokit. Enrichment analysis was performed using Metaboanalyst v4 employing pathway-associated metabolite sets. We established a bioenergetic profile coordinated by the Warburg phenomenon in both groups, but with a massively different outcome that mainly depended upon its group mitochondrial function and redox state. G1 cells are overwhelmed by glycolytic intermediate accumulation due to failing mitochondria, while the functional electron transport chain and improved redox in G2 compensate for Warburg disruption. Both metabolomes reveal the production and abundance of epigenetic modifiers. G1 and G2 metabolomes differ and eventually determine the MDS clinical phenotype, as well as the potential for malignant transformation. |
Τίτλος πηγής δημοσίευσης: | Cancers |
Τόμος/Κεφάλαιο: | 12 |
Τεύχος: | 12 |
Σελίδες: | 3520 |
Θεματική Κατηγορία: | [EL] Κυτταρολογία[EN] Cytology [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) |
Λέξεις-Κλειδιά: | Warburg effect Acute myeloid leukemia Epigenetics Metabolomics Mitochondria Mitochondrial uncoupling Myelodysplastic syndrome Redox Redox ratios |
Κάτοχος πνευματικών δικαιωμάτων: | Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
Ηλεκτρονική διεύθυνση στον εκδότη (link): | https://www.mdpi.com/2072-6694/12/12/3520 |
Ηλεκτρονική διεύθυνση περιοδικού (link) : | https://www.mdpi.com/journal/cancers |
Εμφανίζεται στις συλλογές: | Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο
|