Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Occupational exposure to potentially toxic elements alters gene expression profiles in formal and informal Brazilian workers
Δημιουργός/Συγγραφέας:	Salles, Fernanda Junqueira Frydas, Ilias S. Papaioannou, Nafsika Schultz, Dayna R. Luz, Maciel Santos Rogero, Marcelo Macedo [EL] Σαρηγιάννης, Δημοσθένης[EN] Sarigiannis, Dimosthenis Olympio, Kelly Polido Kaneshiro
Ημερομηνία:	2023
Γλώσσα:	Αγγλικά
ISSN:	00139351
DOI:	10.1016/j.envres.2023.116835
Περίληψη:	Chemical elements, such as toxic metals, have previously demonstrated their ability to alter gene expression in humans and other species. In this study, microarray analysis was used to compare the gene expression profiles of different occupational exposure populations: a) informal workers who perform soldering of jewelry inside their houses (n = 22) in São Paulo (SP) State; and b) formal workers from a steel company (n = 10) in Rio de Janeiro (RJ) state, Brazil. Control participants were recruited from the same neighborhoods without occupational chemical exposure (n = 19 in SP and n = 8 in RJ). A total of 68 blood samples were collected and RNA was extracted and hybridized using an Agilent microarray platform. Data pre-processing, statistical and pathway analysis were performed using GeneSpring software. Different expression was detected by fold-change analysis resulting in 16 up- and 33 down-regulated genes in informal workers compared to the control group. Pathway analysis revealed genes enriched in MAPK, Toll-like receptor, and NF-kappa B signaling pathways, involved in inflammatory and immune responses. In formal workers, 20 up- and 50 down-regulated genes were found related to antimicrobial peptides, defensins, neutrophil degranulation, Fc-gamma receptor-dependent phagocytosis, and pathways associated with atherosclerosis development, which is one of the main factors involved in the progression of cardiovascular diseases. The gene IFI27 was the only one commonly differentially expressed between informal and formal workers and is known to be associated with various types of cancer. In conclusion, differences in gene expression related to occupational exposure are mainly associated with inflammation and immune response. Previous research has identified a link between inflammation and immune responses and the development of chronic diseases, suggesting that prolonged occupational exposures to potentially toxic elements in Brazilian metal workers could lead to negative health outcomes. Further analysis should be carried out to investigate its direct effects and to validate causal associations.
Τίτλος πηγής δημοσίευσης:	Environmental Research
Τόμος/Κεφάλαιο:	236
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Γενετική[EN] Genetics [EL] Ανοσολογία[EN] Immunology
Λέξεις-Κλειδιά:	Immune response Inflammation Occupational exposure Toxic elements
EU Grant:	2017/25424-9 2020/00919-8 2018/18391-0 2017/20752-8 2014/50887-4 CNPq #314637/2021-4 32580820.8.0000.5421
EU Grant identifier:	2017/25424-9 2020/00919-8 2018/18391-0 2017/20752-8 2014/50887-4 CNPq #314637/2021-4
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 Elsevier Inc. All rights reserved
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.envres.2023.116835
Σημειώσεις:	This research was funded by “Fundação de Amparo à Pesquisa do Estado de São Paulo,” FAPESP, grant number 2017/25424-9; 2020/00919-8; 2018/18391-0 and 2017/20752-8; 2014/50887-4”. KPKO is the recipient of a scholarship from “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq #314637/2021-4). The authors would like to thank the volunteers who agreed to participate in this investigation. The authors acknowledge the support of the Health Secretariat of Limeira city and the collaboration of all community health agents and coordinators of the basic health units from Limeira. The authors acknowledge the support of the Center for Worker Health and Human Ecology (CESTEH) from the Sergio Arouca National School of Public Health, Oswaldo Cruz Foundation for the sample collection in the city of Volta Redonda. Also, the members of the eXsat (The Human Exposome Research Group) for the aid in sample collection. Supplementary data to this article can be found online at https://doi. org/10.1016/j.envres.2023.116835
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