Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Differentiation of Brucella melitensis field strains from the vaccine strain Rev-1
Δημιουργός/Συγγραφέας:	Noutsios, G.T Papi, R.M. Ekateriniadou, L.V. Minas, A. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A.
Εκδότης:	Wiley-Blackwell Publishing Ltd.
Ημερομηνία:	2008
Γλώσσα:	Αγγλικά
ISSN:	1742-464X
DOI:	10.1111/j.1742-4658.2008.06448.x
Περίληψη:	Past efforts to differentiate the Brucella spp. have been hampered owing to the high genetic homogeneity among Brucella species. The availability of discriminatory molecular tools to inform and assist conventional epidemiological approaches is invaluable in controlling these infections. The hypervariable octameric oligonucleotide finger-printing method was implemented using microsatellite DNA to genotype Br. melitensis. An eight-base pair tandem repeat sequence was revealed in eight genomic loci of the Br. melitensis genome that existed in various, multiple and random repetitions. Specific primers that were previously described were used to amplify these particular loci. The composition of the omp2 locus from two gene copies with differences in their PstI restriction endonuclease sites was studied in 45 field strains of Br. melitensis and was compared with the vaccine strain Rev-1. Out of the nine loci studied for all samples, four exhibit differences and can be used for differentiating and biotyping of Br. melitensis pathogenic stains from the vaccine strain. In addition the digestion products of the omp2 amplicons by PstI enzyme yielded different fragments between vaccine and field strains of Br. melitensis. Hence contagious field strains can be differentiated from the vaccine strain both with HOOF-prints method and RFLP of omp2 gene.
Τίτλος πηγής δημοσίευσης:	The FEBS Journal
Τόμος/Κεφάλαιο:	275
Τεύχος:	s1
Σελίδες:	419-419
Κάτοχος πνευματικών δικαιωμάτων:	© 2008 The Authors Journal compilation © 2008 FEBS
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/journal/119877016/tocgroup
Σημειώσεις:	Abstract Poster Preseantation
Εμφανίζεται στις συλλογές:	Άλλες δράσεις