Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult
Δημιουργός/Συγγραφέας:	Tsolou, Avgi Nelson, Glyn Trachana, Varvara [EL] Χονδρογιάννη, Νίκη[EN] Chondrogianni, Niki Saretzki, Gabriele von Zglinicki, Thomas [EL] Γκόνος, Ευστάθιος[EN] Gonos, Efstathios S.
Εκδότης:	Wiley-blackwell
Τόπος έκδοσης:	Malden
Ημερομηνία:	2012-05
Γλώσσα:	Αγγλικά
ISSN:	1521-6543
DOI:	10.1002/iub.1018
Περίληψη:	The DNA damage response (DDR) orchestrates the recruitment of repair proteins at sites of damage and arrests cell-cycle progression until completion of repair. Upon irreparable damage, DNA damage foci persist (long-lived foci) and this is believed to induce cellular senescence. The resolution of DNA damage foci has previously been shown to depend on proteasomal degradation and various proteasome subunits have been implicated in the DDR. In this study, we aimed to analyze the possible distinct roles of individual proteasome subunits in the DDR. We show that specific 19S subunits respond to DNA damage by increased protein levels and nuclear translocation. Importantly, two 19S subunits, Rpn7 and Rpn11, colocalize with DNA damage foci over their whole lifespan. Although silencing of Rpn11 does not affect foci stability and lifespan, silencing of Rpn7 promotes faster resolution of DNA damage foci following genotoxic insult. For the first time, we provide evidence that Rpn7 silencing specifically decreases the frequencies of long-lived DNA damage foci without, however, affecting the repair rate of short-lived foci. Therefore, we propose that interaction of Rpn7 with DDR foci in situ mediates the protection of DNA damage foci from premature resolution. We suggest that this interaction is involved in enabling cellular senescence following genotoxic insult.
Τίτλος πηγής δημοσίευσης:	Iubmb Life (formerly Biochemistry and Molecular Biology International) (no Longer Published by Taylor & Francis)
Τόμος/Κεφάλαιο:	64
Τεύχος:	5
Σελίδες:	432-442
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	19S proteasome Rpn7 DNA damage response senescence
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Όροι και προϋποθέσεις δικαιωμάτων:	Free access by the publisher. This article appear in "Iubmb Life (formerly Biochemistry and Molecular Biology International), v64, Issue 5, p. 432-442", and can be found at the following address on Wiley Online Library : http://dx.doi.org/10.1002/iub.1018
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://iubmb.onlinelibrary.wiley.com/journal/15216551
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο