Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Comparative qualitative and quantitative analysis of scleroderma (systemic sclerosis) serologic immunoassays
Δημιουργός/Συγγραφέας:	Picha, Lefkothea Pakas, Ioannis [EL] Γκιάλη, Αποστολία[EN] Guialis, Apostolia Moutsopoulos, Haralampos M. Vlachoyiannopoulos, Panayiotis G.
Εκδότης:	Academic Press
Τόπος έκδοσης:	LONDON
Ημερομηνία:	2008-09
Γλώσσα:	Αγγλικά
ISSN:	0896-8411
DOI:	10.1016/j.jaut.2008.07.001
Περίληψη:	The heterogeneity of autoantibody specificities occurring in sera from patients with systemic sclerosis (SSc) raised the necessity of developing various methodologies for their detection. A cohort of 150 SSc patients were selected and tested by Indirect Immunofluorescence (IIF), Counterimmunoelectrophoresis (CIE), Immunoblot (IB) using various extracts as antigenic source and RNA precipitation. By preparing a nuclear (IB-nuclear) and a metaphase chromosomal-enriched extract (IB-MC-pellet) from HeLa cells as well as a nucleolar (IB-nucleolar) and a histone (IB-histone) extract from rat liver nuclei, we assessed their sensitivity and specificity for anti-Topo 1, anti-U3RNP, anti-H-1, anti-snRNPs antibodies and ACA. IB-nuclear revealed the highest frequency of anti-Topo I antibodies, while CIE, IB-nucleolar and IB-MC-pellet, when compared to IB-nuclear showed a sensitivity of 89%, 87% and 95%, respectively. IB-MC-pellet was unique for ACA recognition, while IB-nucleolar and IB-MC-pellet showed excellent sensitivity for anti-U3RNP and anti-H, antibody detection. We conclude that IB-nuclear is a highly sensitive system for anti Topo I antibodies determination, but CIE reveals a good sensitivity to be used as a first screening test. IB-nucleolar or IB-MC-pellet are important techniques to detect the variety of antibodies to nucleolus and chromatin-related constituents. A novel specificity against a 28 kD nucleolar protein, non-associated with RNAs is also presented.
Τίτλος πηγής δημοσίευσης:	Journal of Autoimmunity
Τόμος/Κεφάλαιο:	31
Τεύχος:	2
Σελίδες:	166-174
Θεματική Κατηγορία:	[EL] Ανοσολογία[EN] Immunology
Λέξεις-Κλειδιά:	Antigenic source Autoantibodies Immunoblot Systemic sclerosis (scleroderma)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2008 Elsevier Ltd. All rights reserved.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/yjaut
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο