Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Validation of biomarkers for the study of environmental carcinogens: a review
Δημιουργός/Συγγραφέας:	Gallo, Valentina Khan, Aneire Gonzales, Carlos Phillips, David H. Schoket, Bernadette Gyorffy, Erika Anna, Livia Kovacs, Katalin Moller, Peter Loft, Steffen [EL] Κυρτόπουλος, Σωτήριος Α.[EN] Kyrtopoulos, Soterios A. Matullo, Giuseppe Vineis, Paolo
Εκδότης:	Informa Healthcare
Τόπος έκδοσης:	LONDON
Ημερομηνία:	2008
Γλώσσα:	Αγγλικά
ISSN:	1354-750X
DOI:	10.1080/13547500802054611
Περίληψη:	There is a need for validation of biomarkers. Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA. A systematic literature search in PubMed was performed. Information on the variability and reliability of the laboratory tests used for biomarkers measurements was collected. For the evaluation of the evidence on validation we referred to the ACCE criteria. Little is known about intraindividual variation of DNA adduct measurements, but measurements have a good repeatability irrespective of the technique used for their identification; reproducibility improved after the correction for a laboratory factor. A high-sensitivity method is available for the measurement of 1-hydroxypyrene in urine. There is consensus on validation of biomarkers of oxidative damage DNA based on the comet assay and chromatographic measurement in blood while urinary measurements by chromatographic assays are well validated, and ELISA-based assays appear to lack specificity. Immunoassays for the quantification of adducts of N-nitroso compounds are useful for large epidemiological studies, given their sensitivity, the small amount of DNA required and their potential for rapid and high-throughput analysis.
Τίτλος πηγής δημοσίευσης:	Biomarkers
Τόμος/Κεφάλαιο:	13
Τεύχος:	5
Σελίδες:	505-534
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Τοξικολογία[EN] Toxicology. Poisons
Λέξεις-Κλειδιά:	validation DNA adducts 1-hydroxypyrene oxidative damage to DNA N-nitroso compounds environmental carcinogenesis
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© INFORMA HEALTHCARE
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.tandf.co.uk/journals/titles/1354750X.asp
Σημειώσεις:	Review
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο