Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Association between Transcriptional Activity, Local Chromatin Structure, and the Efficiencies of Both Subpathways of Nucleotide Excision Repair of Melphalan Adducts
Δημιουργός/Συγγραφέας:	Episkopou, Hara [EL] Κυρτόπουλος, Σωτήριος Α.[EN] Kyrtopoulos, Soterios A. Sfikakis, Petros P. Fousteri, Maria Dimopoulos, Meletios A. Mullenders, Leon H. F. [EL] Σουλιώτης, Βασίλης Λ.[EN] Souliotis, Vassilis L.
Εκδότης:	American Association for Cancer Research (A A C R)
Τόπος έκδοσης:	PHILADELPHIA
Ημερομηνία:	2009-05-15
Γλώσσα:	Αγγλικά
ISSN:	0008-5472
DOI:	10.1158/0008-5472.CAN-08-3489
Περίληψη:	The repair of melphalan-induced N-alkylpurine monoadducts and interstrand cross-links was examined in different repair backgrounds, focusing on four genes (beta-actin, p53, N-ras, and delta-globin) with dissimilar transcription activities. Adducts were found to be substrates for both global genome repair (GGR) and transcription-coupled repair (TCR), with TCR being less efficient than GGR. In nucleotide excision repair-deficient cells, adducts accumulated to similar levels in all four genes. The repair efficiency in different gene loci varied in a qualitatively and quantitatively similar way in both GGR-deficient and TCR-deficient backgrounds and correlated with transcriptional activity and local chromatin condensation. No strand-specific repair was found in GGR(+)/TCR(+) cells, implying that GGR dominated. Adducts were lost over two sharply demarcated phases: a rapid phase resulting in the removal within 1 hour of up to similar to 80% of the adducts, and a subsequent phase with t(1/2) similar to 36 to 48 hours. Following pretreatment. of cells with alpha-amanitin, the rate of transcription, the state of chromatin condensation, and the repair efficiencies (both TCR and GGR) of the transcribed beta-actin, p53, and N-ras genes became similar to those of the nontranscribed delta-globin gene. In conclusion, a continuous, parallel variation of the state of transcription and local chromatin condensation, on one hand, and the rates of both GGR and TCR, on the other hand, have been shown.
Τίτλος πηγής δημοσίευσης:	Cancer Research
Τόμος/Κεφάλαιο:	69
Τεύχος:	10
Σελίδες:	4424-4433
Θεματική Κατηγορία:	[EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© AMER ASSOC CANCER RESEARCH
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://cancerres.aacrjournals.org/
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο