Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Oncogenic RAS alters the global and gene-specific histone modification pattern during epithelial-mesenchymal transition in colorectal carcinoma cells
Δημιουργός/Συγγραφέας:	Pelaez, Ignacio Mazon Kalogeropoulou, Margarita Ferraro, Angelo Voulgari, Angeliki Pankotai, Tibor Boros, Imre [EL] Πίντζας, Αλέξανδρος[EN] Pintzas, Alexander
Εκδότης:	Pergamon
Τόπος έκδοσης:	OXFORD
Ημερομηνία:	2010-06
Γλώσσα:	Αγγλικά
ISSN:	1357-2725
DOI:	10.1016/j.biocel.2010.01.024
Περίληψη:	The presence of different forms of histone covalent modifications, such as phosphorylation, acetylation and methylation in localized promoter regions are markers for chromatin packing and transcription. Activation of RAS signalling pathways through oncogenic RAS mutations is a hallmark of colorectal cancer Overexpression of Harvey-Ras oncogene induces epithelial-mesenchymal transition (EMT) in Caco-2 cells. We focused on the role of epigenetic modifications of histone H3 and its dependence on RAS signal transduction pathways and oncogenic transformation Using cell lines stably overexpressing oncogenic Harvey-RAS with EMT phenotype, we studied the acquired changes in the H3 histone modification patterns Two genes show Inverse protein expression patterns after Ha-RAS overexpression Cyclin D1, a cell cycle-related gene, and the EMT marker-gene E-cadherin We report that these two genes demonstrate matching inverse histone repression patterns on their promoter, while histone markers associated with an active state of genes were affected by the RAS-activated signalling pathway MEK-ERK-MSK1 Furthermore, we show that though the level of methyltransferases enzymes was increased, the status of H3 three-methylation at lysine 27 (H3K27me(3)), associated with gene repression on the promoter of Cyclin D1, was lower Together, these results suggest that histone covalent modifications can be affected by oncogenic RAS pathways to regulate the expression of target genes like Cycl in D1 or E-caclherin and that the dynamic balance of opposing histone-modifying enzymes is critical for the regulation of cell proliferation
Τίτλος πηγής δημοσίευσης:	International Journal of Biochemistry & Cell Biology, the (formerly Known as International Journal of Biochemistry)
Τόμος/Κεφάλαιο:	42
Τεύχος:	6
Σελίδες:	911-920
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	Histone modifications RAS EMT Colon cancer MSK1
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2010 Elsevier Ltd All rights reserved.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/biocel
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο