Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Nuclear Erythroid Factor 2-mediated Proteasome Activation Delays Senescence in Human Fibroblasts
Δημιουργός/Συγγραφέας:	Kapeta, Suzanne [EL] Χονδρογιάννη, Νίκη[EN] Chondrogianni, Niki [EL] Γκόνος, Ευστάθιος[EN] Gonos, Efstathios S.
Εκδότης:	American Society for Biochemistry and Molecular Biology, Inc.
Τόπος έκδοσης:	BETHESDA
Ημερομηνία:	2010-03-12
Γλώσσα:	Αγγλικά
ISSN:	0021-9258
DOI:	10.1074/jbc.M109.031575
Περίληψη:	Replicative senescence in human fibroblasts is accompanied with alterations of various biological processes, including the impaired function of the proteasome. The proteasome is responsible for the removal of both normal and damaged proteins. Due to its latter function, proteasome is also considered a representative secondary antioxidant cellular mechanism. Nrf2 is a basic transcription factor responsible for the regulation of the cellular antioxidant response that has also been shown to regulate several proteasome subunits in mice. We have established in this study the proteasome-related function of Nrf2 in human fibroblasts undergoing replicative senescence. We demonstrate that Nrf2 has a declined function in senescence, whereas its silencing leads to premature senescence. However, upon its activation by a novel Nrf2 inducer, elevated levels of proteasome activity and content are recorded only in cell lines possessing a functional Nrf2. Moreover, treatment by the Nrf2 inducer results in the enhanced survival of cells following oxidative stress, whereas continuous treatment leads to lifespan extension of human fibroblasts. Importantly the Nrf2-proteasome axis is functional in terminally senescent cultures as these cells retain their responsiveness to the Nrf2 stimuli. In conclusion, these findings open up new directions for future manipulation of the senescence phenotype.
Τίτλος πηγής δημοσίευσης:	Journal of Biological Chemistry
Τόμος/Κεφάλαιο:	285
Τεύχος:	11
Σελίδες:	8171-8184
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.jbc.org/
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο