Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Time-series analysis of gene expression profiles induced by nitrosamides and nitrosamines elucidates modes of action underlying their genotoxicity in human colon cells
Δημιουργός/Συγγραφέας:	Hebels, Dennie G. A. J. Brauers, Karen J. J. van Herwijnen, Marcel H. M. [EL] Γεωργιάδης, Παναγιώτης[EN] Georgiadis, Panagiotis [EL] Κυρτόπουλος, Σωτήριος Α.[EN] Kyrtopoulos, Soterios A. Kleinjans, Jos C. S. de Kok, Theo M. C. M.
Εκδότης:	Elsevier Ireland Limited
Τόπος έκδοσης:	CLARE
Ημερομηνία:	2011-12-15
Γλώσσα:	Αγγλικά
ISSN:	0378-4274
DOI:	10.1016/j.toxlet.2011.09.012
Περίληψη:	N-nitroso compounds (NOCs) may represent a carcinogenic risk to humans following endogenous colonic nitrosation processes. We used the colon adenocarcinoma cell line Caco-2 to investigate transcriptomic changes at three time points (1, 6, 24 h) following exposure to genotoxic concentrations of six different NOCs (two nitrosamides, four nitrosamines) with the purpose of identifying biological processes that may play a part in the carcinogenicity of these compounds. This is especially important for nitrosamide exposure where, in light of their high reactivity, important gene expression modifications may take place early in the exposure. We also analyzed NOC-induced O(6)-methylguanine adducts in relation to transcriptomics since these adducts may influence the expression of genes pivotal in NOC-associated carcinogenicity. Many modified pathways appeared related to DNA damage, cell cycle, apoptosis, growth factor signaling and differentiation, which are linked with carcinogenicity. Nitrosamides showed the strongest response at 1 h of exposure, while nitrosamines had the strongest effect at 6 and 24 h. Additionally, methylation was strongly associated with processes that may contribute to the carcinogenic risk. In summary, we have found that NOC-induced gene expression changes vary over time and that many of the modified pathways and processes indicate a carcinogenic risk associated with NOC exposure.
Τίτλος πηγής δημοσίευσης:	Toxicology Letters
Τόμος/Κεφάλαιο:	207
Τεύχος:	3
Σελίδες:	232-241
Θεματική Κατηγορία:	[EL] Τοξικολογία[EN] Toxicology. Poisons
Λέξεις-Κλειδιά:	Toxicogenomics N-nitroso compounds Time-series Alkylating agents O(6)-methylguanine Colon carcinogenesis
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2011 Elsevier Ireland Ltd. All rights reserved.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/toxlet
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο