Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	A fraction of the transcription factor TAF15 participates in interactions with a subset of the spliceosomal U1 snRNP complex
Δημιουργός/Συγγραφέας:	Leichter, Michael Marko, Marija [EL] Γάνου, Βασιλική[EN] Ganou, Vassiliki [EL] Πατρινού-Γεωργούλα, Μερόπη[EN] Patrinou-Georgoula, Meropi Tora, Laszlo [EL] Γκιάλη, Αποστολία[EN] Guialis, Apostolia
Εκδότης:	Elsevier BV
Τόπος έκδοσης:	AMSTERDAM
Ημερομηνία:	2011-12
Γλώσσα:	Αγγλικά
ISSN:	1570-9639
DOI:	10.1016/j.bbapap.2011.09.008
Περίληψη:	RNA/ssDNA-binding proteins comprise an emerging class of multifunctional proteins with an anticipated role in coupling transcription with RNA processing. We focused here on the highly related transcription factors of the TET sub-class: TLS/FUS, EWS and in particular the least studied member TAF15. An extensive array of immunoprecipitation studies on differentially extracted HeLa nuclei revealed the specific association of TAF15 with the spliceosomal U1 snRNP complex, as deduced by the co-precipitating U1 snRNA, U1-70 K and Sm proteins. Additionally, application of anti-U1 RNP autoantibodies identified TAF15 in the immunoprecipitates. Minor fractions of nuclear TAF15 and U1 snRNP were involved in this association. Pull-down assays using recombinant TAF15 and U1 snRNP-specific proteins (U1-70 K, U1A and U1C) provided in vitro evidence for a direct protein-protein interaction between TAF15 and U1 C, which required the N-terminal domain of TAF15. The ability of TAF15 to directly contact RNA, most likely RNA pol II transcripts, was supported by in vivo UV cross-linking studies in the presence of alpha-amanitin. By all findings, the existence of a functionally discrete subset of U1 snRNP in association with TAF15 was suggested and provided further support for the involvement of U1 snRNP components in early steps of coordinated gene expression.
Τίτλος πηγής δημοσίευσης:	Biochimica Et Biophysica Acta (bba) - Proteins & Proteomics (formerly Known as Biochimica Et Biophysica Acta (bba) - Protein Structure and Molecular Enzymology)
Τόμος/Κεφάλαιο:	1814
Τεύχος:	12
Σελίδες:	1812-1824
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Φυσική[EN] Physics
Λέξεις-Κλειδιά:	TET protein Spliceosomal snRNP RNA pol II transcription RNA splicing RNA/ssDNA-binding protein TAF15-U1 snRNP complex Biophysics
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2011 Elsevier B.V. All rights reserved.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/bbapap
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο