Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	An In Silico Compartmentalized Metabolic Model of Brassica Napus Enables the Systemic Study of Regulatory Aspects of Plant Central Metabolism
Δημιουργός/Συγγραφέας:	Pilalis, Eleftherios [EL] Χατζηιωάννου, Αριστοτέλης[EN] Chatziioannou, Aristotelis Thomasset, Brigitte [EL] Κολίσης, Φραγκίσκος Ν.[EN] Kolisis, Fragiskos N.‏
Εκδότης:	John Wiley & Sons, Inc.
Τόπος έκδοσης:	MALDEN
Ημερομηνία:	2011-07
Γλώσσα:	Αγγλικά
ISSN:	0006-3592
DOI:	10.1002/bit.23107
Περίληψη:	Biochemical network reconstructions represent valuable tools for the computational metabolic modeling of organisms that present a great biotechnological interest. An in silico multi-compartmental model of the central metabolism of the plant Brassica napus (Rapeseed) was constructed, aiming to investigate the metabolic properties of the Brassicaceae family. This family comprises many plants with major importance for the energy and nutrition sector, including the model plant Arabidopsis thaliana. The model utilized as objective function to be subsequently optimized, the biomass production of rapeseed developing embryos, which are characterized by a very high, oil content, up to 60% of biomass weight. In order to study global network properties of seed metabolism, various methods were employed, like Flux Balance Analysis, Principal Component Analysis of the flux space and reaction deletion studies, which simulate the effect of gene knock-out experiments. The model successfully simulated seed growth during the stage of oil accumulation and provided insight, regarding certain aspects of network plasticity, with the emphasis given in lipid biosynthesis regulation.
Τίτλος πηγής δημοσίευσης:	Biotechnology and Bioengineering
Τόμος/Κεφάλαιο:	108
Τεύχος:	7
Σελίδες:	1673-1682
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	metabolic engineering regulatory analysis in silico cellular modeling systems biotechnology plant central metabolism models network plasticity
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2011 Wiley Periodicals, Inc.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/cgi-bin/jhome/71002188
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο