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https://hdl.handle.net/10442/12419
Εξειδίκευση τύπου : | Άρθρο σε επιστημονικό περιοδικό |
Τίτλος: | X-ray sequence ambiguities of Sclerotium rolfsii lectin resolved by mass spectrometry |
Δημιουργός/Συγγραφέας: | Sathisha, G. J. Prakash, Y. K. Subrahmanya Chachadi, V. B. Nagaraja, N. N. Inamdar, S. R. [EL] Λεωνίδας, Δημήτρης Δ.[EN] Leonidas, Demetres D.![semantics logo](/EIE/image/semantics.png) Savithri, H. S. Swamy, B. M. |
Εκδότης: | Springer Wien |
Τόπος έκδοσης: | NEW YORK |
Ημερομηνία: | 2008-08 |
Γλώσσα: | Αγγλικά |
ISSN: | 0939-4451 |
DOI: | 10.1007/s00726-007-0624-y |
Περίληψη: | X-ray crystallography, although a powerful technique for determining the three-dimensional structure of proteins, poses inherent problems in assigning the primary structure in residues Asp/Asn and Glu/Gln since these cannot be distinguished decisively in the electron density maps. In our recently published X-ray crystal structure of the Sclerotium rolfsii lectin (SRL) at 1.1 angstrom resolution, amino acid sequence was initially deduced from the electron density map and residues Asp/Asn and Glu/Gln were assigned by considering their hydrogen bonding potential within their structural neighborhood. Attempts to verify the sequence by Edman sequencing were not successful as the N terminus of the protein was blocked. Mass spectrometry was applied to verify and resolve the ambiguities in the SRL X-ray crystal structure deduced sequence. From the Matrix assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI TOF-MS) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis of tryptic and chymotryptic peptides of SRL, we could confirm and correct the sequence at five locations with respect to Asp/Asn and Glu/Gln. Analysis data also confirmed the positions of Leu/Ile, Gln/Lys residues and the sequence covering 118 of the total 141 residues accounting to 83.68% of the earlier deduced sequence of SRL. |
Τίτλος πηγής δημοσίευσης: | Amino Acids |
Τόμος/Κεφάλαιο: | 35 |
Τεύχος: | 2 |
Σελίδες: | 309-320 |
Θεματική Κατηγορία: | [EL] Βιοχημεία[EN] Biochemistry![semantics logo](/EIE/image/semantics.png) |
Λέξεις-Κλειδιά: | Sclerotium rolfsii lectin amino acid sequence mass spectrometry protein crystal structure |
Αξιολόγηση από ομότιμους (peer reviewed): | Ναι |
Κάτοχος πνευματικών δικαιωμάτων: | © SPRINGER |
Ηλεκτρονική διεύθυνση περιοδικού (link) : | http://www.springer.at/amino_acids |
ΙΒΦΧΒ: αρχειακή συλλογή: | Ινστιτούτο Οργανικής και Φαρμακευτικής Χημείας (ΙΟΦΧ) (έως 2012) |
Εμφανίζεται στις συλλογές: | Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο
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