Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Spectroscopic and catalytic studies of lipases in ternary hexane–1-propanol–water surfactantless microemulsion systems
Δημιουργός/Συγγραφέας:	[EL] Ζουμπανιώτη, Μαρία[EN] Zoumpanioti, Maria Stamatis, Haralambos [EL] Παπαδημητρίου, Βασιλική[EN] Papadimitriou, Vassiliki [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis
Εκδότης:	Elsevier BV
Ημερομηνία:	2006
Γλώσσα:	Αγγλικά
ISSN:	0927-7765
DOI:	10.1016/j.colsurfb.2005.11.012
Περίληψη:	A series of water-in-oil microemulsion systems formulated without surfactant were used to solubilize lipases from Rhizomucor miehei and Candida antarctica B. The effect of the system’s composition on the velocity of enzymic reactions was investigated following a model esterification reaction. The interaction between enzymes and the microemulsion environment was studied by steady state fluorescence spectroscopy. The site of localization of the enzyme within the different microdomains of the dispersed phase was investigated by applying the fluorescence energy transfer technique. To determine the properties of the interface between water and organic solvent of the surfactantless microemulsion systems the Electron Paramagnetic Resonance (EPR) spectroscopic technique was applied. The results indicated that even at low water content, water-rich structures are formed. This was confirmed by conductivity measurements. By the addition of enzyme it was observed that when the aqueous phase of the surfactantless microemulsion systems exceeds 2% (v/v) the enzyme retains its catalytic activity, as it is located within the water pools that protect it from the organic solvent. These confined water phases show a propanol rich interface with hexane and their structure depends on the system’s composition.
Τίτλος πηγής δημοσίευσης:	Colloids and Surfaces B: Biointerfaces
Τόμος/Κεφάλαιο:	47
Τεύχος:	1
Σελίδες:	1-9
Θεματική Κατηγορία:	[EL] Επιστήμη[EN] Science
Λέξεις-Κλειδιά:	Biocatalysis Microemulsion Lipase Spin probe Energy transfer
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2005 Elsevier B.V. All rights reserved © 2005 Elsevier B.V. All rights reserved
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/colsurfb
Σημειώσεις:	Ερευνητικη ομάδα ΕΙΕ: biomimics Ερευνητικη ομάδα ΕΙΕ: enzymology This article has been published in the journal, Colloids and Surfaces B: Biointerfaces [© Elsevier]. The definitive version is available at http://dx.doi.org/10.1016/j.colsurfb.2005.11.012
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο