Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide
Δημιουργός/Συγγραφέας:	Blattner, Christian [EL] Ζουμπανιώτη, Μαρία[EN] Zoumpanioti, Maria Kr¨oner, J¨urgen Schmeer, Georg [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis Kunz, Werner
Εκδότης:	Elsevier BV
Ημερομηνία:	2006
Γλώσσα:	Αγγλικά
ISSN:	0896-8446
DOI:	10.1016/j.supflu.2005.06.007
Περίληψη:	Lipases from Candida antarctica and Mucor miehei were encapsulated in lecithin water-in-oil (w/o) microemulsion-based organogels (MBG). These gels were formulated with either hydroxypropylmethyl cellulose (HPMC) or gelatin. The esterification of lauric acid and 1-propanol catalyzed by these MBGs was examined in supercritical carbon dioxide (scCO2; 35◦C, 110 bar) as solvent for the substrates. The results were compared to those obtained with the reference substrate solvent isooctane. It turned out that the initial rates of this model reaction in scCO2 were higher than those observed in the reference system. Various parameters affecting the biocatalysis such as pressure, alcohol and acid chain length, and gel composition were investigated. Kinetic studies showed that the ester synthesis catalyzed by the immobilized C. antarctica lipase occurs via a Ping Pong Bi Bi mechanism in which only inhibition by excess of alcohol was identified. Values of all kinetic parameters were determined. In addition, experiments on the reusability of these gels in scCO2 were carried out and the state of water within the organogel was examined with the help of differential scanning calorimetry. The present study shows that biocatalysis using MBGs in scCO2 is a promising alternative to other bioconversion processes.
Τίτλος πηγής δημοσίευσης:	The Journal of Supercritical Fluids
Τόμος/Κεφάλαιο:	36
Τεύχος:	3
Σελίδες:	182-193
Θεματική Κατηγορία:	[EL] Επιστήμη[EN] Science
Λέξεις-Κλειδιά:	Microemulsion Organogels Lipases Enzymatic reaction Supercritical CO2
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2005 Elsevier B.V. All rights reserved © 2005 Elsevier B.V. All rights reserved
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/supflu
Σημειώσεις:	Ερευνητικη ομάδα ΕΙΕ: biomimics Ερευνητικη ομάδα ΕΙΕ: enzymology This article has been published in the journal, The Journal of Supercritical Fluids [© Elsevier]. The definitive version is available at http://dx.doi.org/10.1016/j.supflu.2005.06.007
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο