Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Lipase biocatalytic processes in surfactant free microemulsion-like ternary systems and related organogels
Δημιουργός/Συγγραφέας:	[EL] Ζουμπανιώτη, Μαρία[EN] Zoumpanioti, Maria Karali, M. [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis Stamatis, H.
Εκδότης:	Elsevier Inc.
Ημερομηνία:	2006
Γλώσσα:	Αγγλικά
ISSN:	0141-0229
Περίληψη:	Lipases from Rhizomucor miehei (RmL) and Candida antartcica (CaL-B) entrapped in surfactant free microemulsion-like ternary systems consisting of n-hexane, short chain alcohols (1-propanol or 2-methyl-2-propanol) and water, keep their catalytic activity at 30 ◦C, catalyzing efficiently the esterification of fatty acids or natural phenolic acids including cinnamic acid derivatives. High conversions (up to 95%) were obtained with both lipases entrapped in various ternary systems. Lipases catalytic efficiency and stability was influenced by the composition of the ternary systems used. Hydroxypropylmethyl cellulose (HPMC) microemulsions-based organogels (MBGs) formulated with various surfactant free microemulsion-like systems were used as a matrix for enzyme immobilization. These lipase-containing MBGs prove to be a novel solid-phase catalyst for use, under mild conditions, in non-polar solvents as well as in solvent free systems. CaL-B immobilized in surfactant free MBGs keep its catalytic activity for a storage period of 8 months, while the corresponding residual activity of immobilized RmL was decreased by 50%. The stability of enzymes in such surfactant free MBGs is higher than that observed in MBGs formulated with surfactant based w/o microemulsions. Lipases-containing surfactant free MBGs were used in seven consecutive batch syntheses of fatty esters with only 10–15% loss of activity.
Τίτλος πηγής δημοσίευσης:	Enzyme and Microbial Technology
Τόμος/Κεφάλαιο:	39
Τεύχος:	4
Σελίδες:	531-539
Θεματική Κατηγορία:	[EL] Επιστήμη[EN] Science [EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	Surfactant free microemulsions Organogels Lipase Esterification Immobilization Stability Phenolic acids
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	Copyright @ Elsevier
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/journal/enzyme-and-microbial-technology/vol/39/issue/4
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/enzmictec
Σημειώσεις:	Ερευνητικη ομάδα ΕΙΕ: biomimics Ερευνητικη ομάδα ΕΙΕ: enzymology
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο