Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Small nanosized poly(vinyl benzyl trimethylammonium chloride) based polyplexes for siRNA delivery
Δημιουργός/Συγγραφέας:	Lou B. Beztsinna N. Mountrichas G. van den Dikkenberg J.B. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Hennink W.E.
Εκδότης:	Elsevier B.V.
Ημερομηνία:	2017
Γλώσσα:	Αγγλικά
ISSN:	0378-5173
DOI:	10.1016/j.ijpharm.2017.03.036
Άλλο:	PubMed ID: 28336456
Περίληψη:	The success of siRNA gene therapy requires the availability of safe and efficient delivery systems. In the present study, we investigated poly(vinyl benzyl trimethylammonium chloride) (PVTC) and its block copolymer with poly(oligo(ethyleneglycol) methacrylate) (POEGMA) as delivery vector for siRNA. Small polyplexes ranging from 8 to 25 nm in diameter were formed in aqueous solution by spontaneous self-assembly of both the homopolymer and block copolymer with siRNA and the formed particles were stable at physiological ionic strength. It was shown that when human ovarian adenocarcinoma cells were transfected, siRNA polyplexes based on PVTC (40 kDa) and PVTC-POEGMA-4 (PP4, 34 kDa) efficiently induced luciferase gene silencing to the same extent as the formulation based on a commercial lipid (Lipofectamine®) (∼80%), and showed higher gene silencing than the linear polyethylenimine formulation linear polyethylenimine (∼35%). Importantly, the POEGMA block polymers displayed a significantly lower cytotoxicity as compared to L-pEI. siRNA polyplexes based on the block polymers displayed high cellular uptake resulting in ∼50% silencing of luciferase expression also in the presence of serum. These results demonstrate that PVTC-based polymers are promising siRNA delivery vectors.
Τίτλος πηγής δημοσίευσης:	International Journal of Pharmaceutics
Τόμος/Κεφάλαιο:	525
Τεύχος:	2
Σελίδες:	388-396
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Λέξεις-Κλειδιά:	Gene therapy Polymeric gene delivery Polyplexes Quaternized polymer siRNA
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2017 Elsevier B.V.
Σημειώσεις:	China Scholarship Council, CSC
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο