Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Lysozyme complexes with thermo- and pH-responsive PNIPAM-b-PAA block copolymer
Δημιουργός/Συγγραφέας:	Pippa N. Meristoudi A. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Demetzos C.
Εκδότης:	Springer Netherlands
Ημερομηνία:	2017
Γλώσσα:	Αγγλικά
ISSN:	1388-0764
DOI:	10.1007/s11051-017-3782-1
Περίληψη:	Lysozyme is an enzyme responsible for the damage of bacterial cell walls and is abundant in a number of secretions such as tears and human milk. In the present study, we investigated the structure, the physicochemical characteristics, and the temperature-responsiveness of lysozyme complexes with poly(N-isopropylacrylamide)-b-poly(acrylic acid) block polyelectrolyte in aqueous media. A gamut of light-scattering techniques and fluorescence spectroscopy were used in order to examine the complexation process, as well as the structure, solution behavior, and temperature response of the nanosized complexes. The concentration of copolymer polyelectrolyte was kept constant. The values of the scattering intensity, I90, which is proportional to the mass of the species in solution, increased gradually as a function of CLYS, providing proof of the occurring complexation, while the size of the nanostructures decreased. The structure of the complexes became more open as the CLYS increased. The increase of the salinity did not affect the structural characteristics of the supramolecular nanoparticulate aggregates. On the other hand, the physicochemical and structural characteristics of the complexes changed upon increasing temperature, and the changes depended on the initial ratio block polyelectrolyte/lysozyme. The knowledge on developing block polyelectrolyte/protein complexes through electrostatic interactions, obtained from this investigation, may be applied to the design of nutraceuticals.
Τίτλος πηγής δημοσίευσης:	Journal of Nanoparticle Research
Τόμος/Κεφάλαιο:	19
Τεύχος:	2
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	Block polyelectrolyte Complexation process Electrostatic interactions Lysozyme Self-assembly Thermoresponsive nanocarriers
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2017, Springer Science+Business Media Dordrecht.
Σημειώσεις:	European Commission, EC; European Social Fund, ESF Authors acknowledge financial support of the work by the NANOMACRO 1129 project which is implemented in the framework of the Operational Program “Education and Life-long Learning” (Action “ARISTEIA I”) and it is co-funded by the European Union (European Social Fund) and by national funds. The authors would like to thank Dr. Aggeliki Siamidi for her help in editing this manuscript.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο