Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Plasma-assisted nanoscale protein patterning on si substrates via colloidal lithography
Δημιουργός/Συγγραφέας:	Malainou A. Tsougeni K. Ellinas K. Petrou P.S. Constantoudis V. [EL] Σαραντοπούλου, Ευαγγελία[EN] Sarantopoulou, Evangelia Awsiuk K. Bernasik A. Budkowski A. Markou A. Panagiotopoulos I. Kakabakos S.E. Gogolides E. Tserepi A.
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	1089-5639
DOI:	10.1021/jp407810x
Άλλο:	PubMed ID: 24180245
Περίληψη:	Selective immobilization of proteins in well-defined patterns on substrates has recently attracted considerable attention as an enabling technology for applications ranging from biosensors and BioMEMS to tissue engineering. In this work, a method is reported for low-cost, large scale and high throughput, selective immobilization of proteins on nanopatterned Si, based on colloidal lithography and plasma processing to define the areas (<300 nm) where proteins are selectively immobilized. A close-packed monolayer of PS microparticles is deposited on oxidized Si and, either after microparticle size reduction or alternatively after metal deposition through the PS close-packed monolayer, is used as etching mask to define SiO2 nanoislands (on Si). C4F8 plasma was used to selectively etch and modify the SiO2 nanoislands while depositing a fluorocarbon layer on the Si surface. The plasma-treated surfaces were chemically characterized in terms of functional group identification through XPS analysis and reaction with specific molecules. Highly selective protein immobilization mainly through physical adsorption on SiO2 nanoislands and not on surrounding Si was observed after C4F8 plasma-induced chemical modification of the substrate. The thickness of the immobilized protein monolayer was estimated by means of AFM image analysis. The method reported herein constitutes a cost-efficient route toward rapid, large surface, and high-density patterning of biomolecules on solid supports that can be easily applied in BioMEMS or microanalytical systems.
Τίτλος πηγής δημοσίευσης:	Journal of Physical Chemistry A
Τόμος/Κεφάλαιο:	117
Τεύχος:	50
Σελίδες:	13743-13751
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Εφαρμοσμένη οπτική. Φωτονική[EN] Applied optics. Photonics
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2013 American Chemical Society.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο