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https://hdl.handle.net/10442/17287
Εξειδίκευση τύπου : | Άρθρο σε επιστημονικό περιοδικό |
Τίτλος: | Enhanced Catalytic Performance of Trichoderma reesei Cellulase Immobilized on Magnetic Hierarchical Porous Carbon Nanoparticles |
Δημιουργός/Συγγραφέας: | Papadopoulou A. Zarafeta D. Galanopoulou A.P. Stamatis H. |
Εκδότης: | Springer New York LLC |
Ημερομηνία: | 2019 |
Γλώσσα: | Αγγλικά |
ISSN: | 1572-3887 |
DOI: | 10.1007/s10930-019-09869-w |
Άλλο: | PubMed ID: 31549278 |
Περίληψη: | Cellulase from Trichoderma reesei was immobilized by covalent or non-covalent binding onto magnetic hierarchical porous carbon (MHPC) nanomaterials. The immobilization yield and the enzyme activity were higher when covalent immobilization approach was followed. The covalent immobilization approach leads to higher immobilization yield (up to 96%) and enzyme activity (up to 1.35 U mg−1) compared to the non-covalent cellulase binding. The overall results showed that the thermal, storage and operational stability of the immobilized cellulase was considerably improved compared to the free enzyme. The immobilized cellulose catalyzed the hydrolysis of microcrystalline cellulose up to 6 consecutive successive reaction cycles, with a total operation time of 144 h at 50 °C. The half-life time of the immobilized enzyme in deep eutectic solvents-based media was up to threefold higher compared to the soluble enzyme. The increased pH and temperature tolerance of the immobilized cellulase, as well as the increased operational stability in aqueous and deep eutectic solvents-based media indicate that the use of MHPCs as immobilization nanosupport could expand the catalytic performance of cellulolytic enzymes in various reaction conditions. |
Τίτλος πηγής δημοσίευσης: | Protein Journal |
Τόμος/Κεφάλαιο: | 38 |
Τεύχος: | 6 |
Σελίδες: | 640-648 |
Θεματική Κατηγορία: | [EL] Χημεία (Γενικά)[EN] Chemistry (General) |
Λέξεις-Κλειδιά: | Cellulase Cellulose Deep eutectic solvents Immobilization Magnetic nanomaterials Stabilization |
Αξιολόγηση από ομότιμους (peer reviewed): | Ναι |
Κάτοχος πνευματικών δικαιωμάτων: | © 2019, Springer Science+Business Media, LLC, part of Springer Nature. |
Σημειώσεις: | European Commission, EU; European Social Fund, ESF We acknowledge support of this work by the project MIS 5005434 which has been co-financed by the Operational Program “Human Resources Development, Education and Lifelong Learning” and is co-financed by the European Union (European Social Fund) and Greek national funds. We are grateful to Dr. Michaela Patila and Dr. Konstantinos Spyrou for the synthesis and characterization of MHPC nanoparticles. |
Εμφανίζεται στις συλλογές: | Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο
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