Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Hydroxytyrosol encapsulated in biocompatible water-in-oil microemulsions: How the structure affects in vitro absorption
Δημιουργός/Συγγραφέας:	Mitsou E. Dupin A. Sassi A.H. Monteil J. [EL] Σωτηρούδης, Γιώργος[EN] Sotiroudis, Georgios Leal-Calderon F. [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis
Εκδότης:	Elsevier B.V.
Ημερομηνία:	2019
Γλώσσα:	Αγγλικά
ISSN:	0927-7765
DOI:	10.1016/j.colsurfb.2019.110482
Άλλο:	PubMed ID: 31539752
Περίληψη:	Over the last years, the incorporation of natural antioxidants in food and pharmaceutical formulations has gained attention, delaying or preventing oxidation phenomena in the final products. In order to take full advantage of their properties, protection in special microenvironments is of great importance. The unique features of the natural phenolic compound hydroxytyrosol (HT) - including antioxidant, anti-inflammatory, antiproliferative and cardioprotective properties - have been studied to clarify its mechanism of action. In the present study novel biocompatible water-in-oil (W/O) microemulsions were developed as hosts for HT and subsequently examined for their absorption profile following their oral uptake. The absorption of HT in solution was compared with the encapsulated one in vitro, using a coculture model (Caco-2/TC7 and HT29-MTX cell lines). The systems were structurally characterized by means of Dynamic Light Scattering (DLS) and Electron Paramagnetic Resonance (EPR) techniques. The diameter of the micelles remained unaltered after the incorporation of 678 ppm of HT but the interfacial properties were slightly affected, indicating the involvement of the HT molecules in the surfactant monolayer. EPR was used towards a lipophilic stable free radial, namely galvinoxyl, indicating a high scavenging activity of the systems and encapsulated HT. Finally, after the biocompatibility study of the microemulsions the intestinal absorption of the encapsulated HT was compared with its aqueous solution in vitro. The higher the surfactants’ concentration in the system the lower the HT concentration that penetrated the constructed epithelium, indicating the involvement of the amphiphiles in the antioxidant's absorption and its entrapment in the mucus layer.
Τίτλος πηγής δημοσίευσης:	Colloids and Surfaces B: Biointerfaces
Τόμος/Κεφάλαιο:	184
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General)
Λέξεις-Κλειδιά:	Coculture model Dynamic light scattering (DLS) Electron paramagnetic resonance (EPR) Encapsulation LC–MS/MS Transepithelial electrical resistance (TEER)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2019 Elsevier B.V.
Σημειώσεις:	Agence Nationale de la Recherche, ANR: 07-CARN-009-01; Institut National de la Santé et de la Recherche Médicale, Inserm; General Secretariat for Research and Technology, GSRT; Hellenic Foundation for Research and Innovation, HFRI: 186565/Ι2/1-11-2017. This project was funded by the LISA Carnot Institute (Lipids for health and industry) supported by Agence Nationale de la Recherche (ANR- 07-CARN-009-01 ). Evgenia Mitsou acknowledges Hellenic Foundation for Research and Innovation (HFRI 186565/Ι2/1-11-2017 ) and General Secretariat of Research and Technology (GSRT) for supporting this work under grant scholarships for doctoral dissertation.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο