Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	MTHFR gene variants and non-MALT lymphoma development in primary Sjogren's syndrome
Δημιουργός/Συγγραφέας:	Fragkioudaki S. Nezos A. [EL] Σουλιώτης, Βασίλης Λ.[EN] Souliotis, Vassilis L. Chatziandreou I. Saetta A.A. Drakoulis N. Tzioufas A.G. Voulgarelis M. Sfikakis P.P. Koutsilieris M. Crow M.K. Moutsopoulos H.M. Mavragani C.P.
Εκδότης:	Nature Publishing Group
Ημερομηνία:	2017
Γλώσσα:	Αγγλικά
ISSN:	2045-2322
DOI:	10.1038/s41598-017-07347-w
Άλλο:	PubMed ID: 28779180
Περίληψη:	Primary Sjogren's syndrome (pSS) confers increased risk for non-Hodgkin lymphoma (NHL) development. Two common polymorphisms, the c. 677C > T and c. 1298A > C, of the methylene-tetrahydrofolate reductase (MTHFR) gene, an enzyme essential in DNA synthesis and methylation, have been associated with susceptibility to NHL. Herein, we tested the hypothesis that MTHFR variants contribute to pSS-related lymphomagenesis. 356 pSS patients, of whom 75 had MALT and 19 non-MALT NHL and 600 healthy controls were genotyped for the detection of MTHFR polymorphisms. DNA methylation levels were assessed by pyrosequencing of the LINE-1 retroelement promoter in DNA from 55 salivary gland tissues from pSS patients. DNA double-strand breaks were determined in peripheral blood mononuclear cells from 13 pSS patients, using comet assay. Αnalysis according to lymphoma subtype revealed increased frequency of c. 677C > T TT genotype and T allele, as well as reduced prevalence of the c. 1298A > C C allele in the pSS non-MALT group compared to controls and patients without NHL. MTHFR c. 677C > T TT genotype was associated with reduced DNA methylation levels, while MTHFR c. 1298A > C AC genotype with reduced DNA double-strand breaks levels. MTHFR variants may be involved in SS non-MALT NHL development, through contribution to defective DNA methylation and genomic instability.
Τίτλος πηγής δημοσίευσης:	Scientific Reports
Τόμος/Κεφάλαιο:	7
Τεύχος:	1
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2017 The Author(s).
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Gold, Green
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο