Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	A study on the combined effects of carbon and nitrogen source on high added value products synthesis by Nannochloropsis oceanica CCMP1779 using response surface methodology
Δημιουργός/Συγγραφέας:	Savvidou M.G. Lymperopoulou T.V. Mamma D. Balta-Brouma K.P. [EL] Σωτηρούδης, Θεόδωρος Γ.[EN] Sotiroudis, Theodore G. Kekos D. [EL] Κολίσης, Φραγκίσκος Ν.[EN] Kolisis, Fragiskos N.‏
Εκδότης:	Elsevier Ltd
Ημερομηνία:	2017
Γλώσσα:	Αγγλικά
ISSN:	1878-8181
DOI:	10.1016/j.bcab.2017.04.006
Περίληψη:	Response surface methodology (RSM) was used to evaluate the combined effect of the initial carbon (NaHCO3) and nitrogen (NaNO3) source concentration on the synthesis of high added value products by Nannochloropsis oceanica CCMP1779, applying a 22 full-factorial central composite design with four axial points. Biomass and lipid production were influenced by the variations of the tested parameters. Highest biomass production (0.581 g/L) and specific growth rate (0.279 d−1) were obtained at high carbon concentration, while low nitrogen concentration favors the lipids accumulation (highest lipid content 44.65 g/100 g DCW). The fatty acids (FAs) profile of N. oceanica CCMP1779 was dominated by saturated (SFAs) and monounsaturated fatty acids (MUFAs), comprising 88–95% of the total FAs, while polyunsaturated acids (PUFAs) accounted for 5–11%. Palmitic (C16:0), palmitoleic (C16:1 n-7), oleic acid (C18:1 n-9), myristic (C14:0) and eicosapentaenoic acid (C20:5 n-3) (EPA) were the major FAs detected and their content depended on the (NaHCO3) and nitrogen (NaNO3) combination. Optimization of through RSM indicated that a combination of 1.3 g/L NaHCO3 and 0.06 g/L NaNO3 maximizes the FAs accumulation and MUFAs content, while the above conditions have the exact opposite effect on PUFAs and EPA content.
Τίτλος πηγής δημοσίευσης:	Biocatalysis and Agricultural Biotechnology
Τόμος/Κεφάλαιο:	10
Σελίδες:	298-307
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	Carbon and nitrogen source concentration Fatty acids and MUFAs optimization Nannochloropsis oceanica CCMP1779 Response surface methodology
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο