Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Application of cationic polymer micelles for the dispersal of bacterial biofilms
Δημιουργός/Συγγραφέας:	Borisova D. Haladjova E. Kyulavska M. Petrov P. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Stoitsova S. Paunova-Krasteva T.
Εκδότης:	Wiley-VCH Verlag
Ημερομηνία:	2018
Γλώσσα:	Αγγλικά
ISSN:	1618-0240
DOI:	10.1002/elsc.201800040
Περίληψη:	Contamination of surfaces in hospitals and food industry by bacterial biofilms is a serious health risk. Of concern is their resistance to routine antibacterials and disinfectants. This requires the development of novel approaches to biofilm detachment. The study evaluates the effectiveness of cationic polymer micelles (CPMs) against pre-formed biofilms. CPMs based on different polycations were used. The hydrodynamic radius of the particles ranged from 16 to 360 nm. Biofilms of Escherichia coli 420, Pseudomonas aeruginosa PAO1, Staphylococcus aureus 29213 and Bacillus subtilis 168 were cultivated for 24 h then the pre-formed biofilms were treated with the CPMs for 2, 4 or 6 h. Biofilm biomass was evaluated by the crystal violet assay, and live/dead fluorescence test was applied for bacterial viability. The ability of CPMs to interact with pre-formed biofilms of the model strains was evaluated. We observed that the most effective CPMs were those based on poly(2-(dimethylamino)ethyl methacrylate) copolymers which reduced the biofilm biomass three- to four-fold compared with the treatment of the biofilm with water. Significantly reduced vitality of the bacteria in the biofilms was registered by the live/dead stain. The results indicate the applicability of the CPMs for disinfection of biofilm-contaminated surfaces and the treatment of wounds.
Τίτλος πηγής δημοσίευσης:	Engineering in Life Sciences
Τόμος/Κεφάλαιο:	18
Τεύχος:	12
Σελίδες:	943-948
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Λέξεις-Κλειδιά:	Bacterial biofilm dispersal Cationic polymer micelles
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Green
Σημειώσεις:	DFNP-17-129/31.07.2017; Bulgarian Academy of Sciences, BAS This work was funded by a “Program for career development of young scientists and PhD students from Bulgarian Academy of Sciences”, Contract No. DFNP-17-129/31.07.2017.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο