Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Analysis of the machinery and intermediates of the 5hmC-mediated DNA demethylation pathway in aging on samples from the MARKAGE Study
Δημιουργός/Συγγραφέας:	Valentini E. Zampieri M. Malavolta M. Bacalini M.G. Calabrese R. Guastafierro T. Reale A. Franceschi C. Hervonen A. Koller B. Bernhardt J. Eline Slagboom P. Toussaint O. Sikora E. [EL] Γκόνος, Ευστάθιος[EN] Gonos, Efstathios S. Breusing N. Grune T. Jansen E. Dollé M.E.T. Moreno-Villanueva M. Sindlinger T. Bürkle A. Ciccarone F. Caiafa P.
Εκδότης:	Impact Journals LLC
Ημερομηνία:	2016
Γλώσσα:	Αγγλικά
ISSN:	1945-4589
DOI:	10.18632/aging.101022
Άλλο:	PubMed ID: 27587280
Περίληψη:	Gradual changes in the DNA methylation landscape occur throughout aging virtually in all human tissues. A widespread reduction of 5-methylcytosine (5mC), associated with highly reproducible site-specific hypermethylation, characterizes the genome in aging. Therefore, an equilibrium seems to exist between general and directional deregulating events concerning DNA methylation controllers, which may underpin the age-related epigenetic changes. In this context, 5mC-hydroxylases (TET enzymes) are new potential players. In fact, TETs catalyze the stepwise oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), driving the DNA demethylation process based on thymine DNA glycosylase (TDG)-mediated DNA repair pathway. The present paper reports the expression of DNA hydroxymethylation components, the levels of 5hmC and of its derivatives in peripheral blood mononuclear cells of age-stratified donors recruited in several European countries in the context of the EU Project 'MARK-AGE'. The results provide evidence for an age-related decline of TET1, TET3 and TDG gene expression along with a decrease of 5hmC and an accumulation of 5caC. These associations were independent of confounding variables, including recruitment center, gender and leukocyte composition. The observed impairment of 5hmC-mediated DNA demethylation pathway in blood cells may lead to aberrant transcriptional programs in the elderly.
Τίτλος πηγής δημοσίευσης:	Aging
Τόμος/Κεφάλαιο:	8
Τεύχος:	9
Σελίδες:	1896-1922
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	Aging DNA hydroxymethylation TDG TET genes
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Gold, Green
Σημειώσεις:	Seventh Framework Programme, FP7: 200880
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο