Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence
Δημιουργός/Συγγραφέας:	Schindler T. González A. Boopathi R. Roda M.M. Romero-Santacreu L. Wildes A. Porcar L. Martel A. [EL] Θεοδωρακόπουλος, Νίκος[EN] Theodorakopoulos, Nikos Cuesta-López S. Angelov D. Unruh T. Peyrard M.
Εκδότης:	American Physical Society
Ημερομηνία:	2018
Γλώσσα:	Αγγλικά
ISSN:	2470-0045
DOI:	10.1103/PhysRevE.98.042417
Περίληψη:	DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp≈500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain.
Τίτλος πηγής δημοσίευσης:	Physical Review E
Τόμος/Κεφάλαιο:	98
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2018 American Physical Society.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Green
Σημειώσεις:	Deutsche Forschungsgemeinschaft, DFG; Agence Nationale de la Recherche, ANR: ANR-12-BSV5-0017-01, ANR-17-CE11-0019-03; Ministry of Economy, Trade and Industry, METI: BES-2013-065453, EEBB-I-2015-09973, FIS2012-38827; European Regional Development Fund, FEDER We thank the 15ID-D USAXS beamline at the Advanced Photon Source, who kindly provided the glassy carbon standard sample used for the absolute normalization of the SAXS data. T.S. and T.U. gratefully acknowledge the financial support by the Deutsche Forschungsgemeinschaft (DFG) through the Cluster of Excellence Engineering of Advanced Materials (EAM) and GRK 1896 In-Situ Microscopy with Electrons, X-rays and Scanning Probes. A.G. thanks the Ph.D. program of the Institut Laue Langevin for providing the financial support for his thesis. M.M.R., L.R.S., and S.C.L. acknowledge the overall support by the Spanish Ministry of Economy, Industry and Competitiveness (BES-2013-065453, EEBB-I-2015-09973, FIS2012-38827). S.C.L. and UC-154 are grateful for the support of Junta de Castilla y Leon (Spain) Nanofibersafe BU079U16. D.A. acknowledges funding from the Agence Nationale de la Recherche through ANR-12-BSV5-0017-01 “Chrome” and ANR-17-CE11-0019-03 “Chrom3D” grants. N.T. acknowledges support by the project Advanced Materials and Devices (MIS 5002409, Competitiveness, Entrepreneurship and Innovation, NSRF 2014-2020) cofinanced by Greece and the European Regional Development Fund.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο