Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Expression and promoter methylation status of hMLH1, MGMT, APC, and CDH1 genes in patients with colon adenocarcinoma
Δημιουργός/Συγγραφέας:	Michailidi C. Theocharis S. Tsourouflis G. [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki Kouraklis G. Patsouris E. Papavassiliou A.G. Troungos C.
Εκδότης:	SAGE Publications Inc.
Ημερομηνία:	2015
Γλώσσα:	Αγγλικά
ISSN:	1535-3702
DOI:	10.1177/1535370215583800
Άλλο:	PubMed ID: 25908636
Περίληψη:	Colorectal cancer (CRC) is the third most common cancer in men and the second in women worldwide. CRC development is the result of genetic and epigenetic alterations accumulation in the epithelial cells of colon mucosa. In the present study, DNA methylation, an epigenetic event, was evaluated in tumoral and matching normal epithelium in a cohort of 61 CRC patients. The results confirmed and expanded knowledge for the tumor suppressor genes hMLH1, MGMT, APC, and CDH1. Promoter methylation was observed for all the examined genes in different percentage. A total of 71% and 10% of the examined cases were found to be methylated in two or more and in all genes, respectively. mRNA and protein levels were also evaluated. Promoter methylation of hMLH1, MGMT, APC, and CDH1 genes was present at the early stages of tumor’s formation and it could also be detected in the normal mucosa. Correlations of the methylated genes with patient’s age and tumor’s clinicopathological characteristics were also observed. Our findings suggest that DNA methylation is a useful marker for tumor progression monitoring and that promoter methylation in certain genes is associated with more advanced tumor stage, poor differentiation, and metastasis.
Τίτλος πηγής δημοσίευσης:	Experimental Biology and Medicine
Τόμος/Κεφάλαιο:	240
Τεύχος:	12
Σελίδες:	1599-1605
Θεματική Κατηγορία:	[EL] Γενετική[EN] Genetics
Λέξεις-Κλειδιά:	Colon cancer CpG islands epigenetics methylation methylation-specific PCR
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2015, © 2015 by the Society for Experimental Biology and Medicine.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Green
Σημειώσεις:	This work and Dr C. Michailidi were supported by the Stavros Niarchos Foundation, SNF
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο