Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Binding and stabilisation effects of glycodendritic compounds with peanut agglutinin
Δημιουργός/Συγγραφέας:	Sleiman M.H. Csonka Ró. [EL] Αρμπέζ-Ζιντρ, Σεσίλ[EN] Arbez-Gindre, Cecile [EL] Χαιρόπουλος, Γεώργιος Α.[EN] Heropoulos, George A. [EL] Καλογεροπούλου, Θεοδώρα[EN] Calogeropoulou, Theodora Signorelli M. Schiraldi A. [EL] Στηλ, Μπάρυ[EN] Steele, Barry R. Fessas D. [EL] Μίχα-Σκρέττα, Μαρία[EN] Micha-Screttas, Maria
Εκδότης:	Elsevier
Ημερομηνία:	2015
Γλώσσα:	Αγγλικά
ISSN:	0141-8130
DOI:	10.1016/j.ijbiomac.2015.07.036
Άλλο:	PubMed ID: 26206739
Περίληψη:	A number of new polyhydroxy-dendritic structures have been constructed from a few basic modules. The cores were derived from N-. tert(butyloxycarbonyl)tris[(propargyloxy)methyl]aminomethane, N,N′-bis-1,3-(tris-(propargyloxymethyl)methyl)-5-(hydroxymethyl)isophthalamide, and N,N′, N″-tris-1,3,5-(tris-(propargyloxymethyl)methyl)-1,3,5-benzene tricarboxamide while the terminal groups were derived from β-azido-galactose and β-azido-lactose leading to six new glycodendrimeric compounds with up to 63 hydroxyl groups on the periphery. The binding ability of the new compounds to peanut agglutinin (PNA), a galactose recognizing lectin from Arachis hypogaea, was investigated by nano-Isothermal Titration Calorimetry and nano-Differential Scanning Calorimetry. We found that the compounds had stronger stabilising effect on the macromolecules compared to the corresponding sugars. The interaction between lectin and the glycodendrimeric unit is entropically driven with only a low enthalpic contribution. A trend was found with increasing number of carbohydrates that is strongly influenced by the steric constraints of the ligands. Our results indicate the significance of multivalency and size control in the successful design of lectin inhibitors.
Τίτλος πηγής δημοσίευσης:	International Journal of Biological Macromolecules
Τόμος/Κεφάλαιο:	80
Σελίδες:	692-701
Θεματική Κατηγορία:	[EL] Φαρμακευτική χημεία[EN] Pharmaceutical chemistry [EL] Οργανική χημεία[EN] Organic chemistry
Λέξεις-Κλειδιά:	Arachis hypogaea Calorimetry DSC Glycodendrimer ITC Lectin Multivalency PNA
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2015 Elsevier B.V.
Σημειώσεις:	European Commission, EC: GA 245866, MTKD-CT-2004-014399; European Cooperation in Science and Technology, COST: TD0802. This work was supported by the European Commission through the Marie-Curie Transfer of Knowledge Project “SOPHOLIDES” (MTKD-CT-2004-014399) and the REGPOT-2009-1 Project “ARCADE” (GA 245866), and by COST Action TD0802.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο