Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Investigation of the interactions of silibinin with 2-hydroxypropyl-β-cyclodextrin through biophysical techniques and computational methods
Δημιουργός/Συγγραφέας:	Kellici T.F. Ntountaniotis D. Leonis G. Chatziathanasiadou M. Chatzikonstantinou A.V. Becker-Baldus J. Glaubitz C. Tzakos A.G. Viras K. Chatzigeorgiou P. Tzimas S. Kefala E. Valsami G. Archontaki H. [EL] Παπαδόπουλος, Μάνθος Γ.[EN] Papadopoulos, Manthos G. [EL] Μαυρομούστακος, Θωμάς[EN] Mavromoustakos, Thomas
Εκδότης:	American Chemical Society
Ημερομηνία:	2015
Γλώσσα:	Αγγλικά
ISSN:	1543-8384
DOI:	10.1021/mp5008053
Άλλο:	PubMed ID: 25665128
Περίληψη:	Cyclodextrins (CDs) are a well-known class of supermolecules that have been widely used to protect drugs against conjugation and metabolic inactivation as well as to enhance the aqueous solubility and hence to ameliorate the oral bioavailability of sparingly soluble drug molecules. The hepatoprotectant drug silibinin can be incorporated into CDs, and here we elucidate the interaction between the drug and the host at the molecular level. The complexation product of silibinin with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) is characterized by Differential Scanning Calorimetry, mass spectrometry, solid and liquid high-resolution NMR spectroscopy. The chemical shift changes using 13C CP/MAS on the complexing of the guest with the host provided significant information on the molecular interactions, and they were in agreement with the 2D NOESY results. These results point out that in both solid and liquid forms, the drug is engulfed and interacts with HP-β-CD in identical manner. Molecular dynamics calculations have been performed to examine the thermodynamic characteristics associated with the silibinin-HP-β-CD interactions and to study the stability of the complex. To approximate the physiological conditions, the aqueous solubility and dissolution characteristics of the complex at pH states simulating those of the upper gastrointestinal tract have been applied. To evaluate the antiproliferative activity of silibinin-HP-β-CD complex comparatively to silibinin in MCF-7 human cancer cells, MTT assays have been performed. (Graph Presented).
Τίτλος πηγής δημοσίευσης:	Molecular Pharmaceutics
Τόμος/Κεφάλαιο:	12
Τεύχος:	3
Σελίδες:	954-965
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General)
Λέξεις-Κλειδιά:	2-hydroxypropyl-β-cyclodextrin differential scanning calorimetry molecular dynamics MTT assay NMR spectroscopy silibinin or silybin
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2015 American Chemical Society.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο