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Εξειδίκευση τύπου : Άρθρο σε επιστημονικό περιοδικό
Τίτλος: Phycocyanobilin, a bioactive tetrapyrrolic compound of blue-green alga Spirulina, binds with high affinity and competes with bilirubin for binding on human serum albumin
Δημιουργός/Συγγραφέας: Minic S.L.
Milcic M.
Stanic-Vucinic D.
Radibratovic M.
[EL] Σωτηρούδης, Θεόδωρος Γ.[EN] Sotiroudis, Theodore G.semantics logo
Nikolic M.R.
Velickovic T.C.
Εκδότης: Royal Society of Chemistry
Ημερομηνία: 2015
Γλώσσα: Αγγλικά
ISSN: 2046-2069
DOI: 10.1039/c5ra05534b
Περίληψη: Human serum albumin (HSA) is an important regulator of the pharmacokinetic properties of bioactive compounds. Phycocyanobilin is a blue tetrapyrrole chromophore of C-phycocyanin with proven health-promoting activities. Despite its structural similarity to bilirubin, the conformation it adopts in aqueous solution is different and the pigment is more soluble than bilirubin. The aim of our study was to examine binding of phycocyanobilin for HSA and to investigate its competition with bilirubin. Based on a computational approach, we demonstrated two putative high-affinity binding pockets on HSA of virtually identical energies for the neutral and anion forms of bilirubin, but with slightly favorable predictions for anion forms of phycocyanobilin. Computational prediction of phycocyanobilin pKa values suggested a monoanion form to be the most stable form at physiological conditions. The computationally predicted binding sites for phycocyanobilin were identical to the two previously identified binding sites for bilirubin (subdomains IB and IIA). Results obtained by protein and pigment fluorescence measurements, circular dichroism, and competition experiments confirmed high affinity (binding constant of 2.2 × 106 M-1 at 25 °C), stereo-selective binding of phycocyanobilin M-conformer to HSA and its competition with bilirubin, warfarin and hemin. Our experimental data confirm that phycocyanobilin binds to IB and IIA binding site of HSA with an affinity similar to bilirubin. In conditions characterized by an increased bilirubin plasma concentration, or intake of drugs binding to IB or IIA binding site, pharmacokinetics of phycocyanobilin may also be changed.
Τίτλος πηγής δημοσίευσης: RSC Advances
Τόμος/Κεφάλαιο: 5
Τεύχος: 76
Σελίδες: 61787-61798
Θεματική Κατηγορία: [EL] Βιολογία (Γενικά)[EN] Biology (General)semantics logo
Αξιολόγηση από ομότιμους (peer reviewed): Ναι
Κάτοχος πνευματικών δικαιωμάτων: © The Royal Society of Chemistry 2015.
Όροι και προϋποθέσεις δικαιωμάτων: All Open Access, Green
Σημειώσεις: Seventh Framework Programme, FP7: 256716
Εμφανίζεται στις συλλογές:Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο

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