Εξειδίκευση τύπου :	Ανακοίνωση σε συνέδριο
Τίτλος:	An Entropy-based statistical workflow provides noise-minimizing biological annotation for
Δημιουργός/Συγγραφέας:	Aging M. Koutsandreas T. Valavanis I. Pilalis E. [EL] Χατζηιωάννου, Αριστοτέλης[EN] Chatziioannou, Aristotelis
Επιμελητής έκδοσης:	Chen L. Zhang X.-S. Wu L.-Y. Wang Y.
Χορηγός :	Academy of Mathematics and Systems Sciences of CAS (AMSS) National Natural Science Foundation of China (NSFC) Qingdao Institute of Bioenergy and Bioprocess Technology of CAS (QIBEBT) Qingdao University Shanghai Institutes for Biological Sciences of CAS (SIBS)
Εκδότης:	IEEE Computer Society
Ημερομηνία:	2014
Γλώσσα:	Αγγλικά
ISBN:	9781479972944
ISSN:	2325-0704
DOI:	10.1109/ISB.2014.6990749
Περίληψη:	This study aims to expand the efficiency of the interpretation concerning the aging process, by exploring a broad gene set, derived from the analysis of an integrative transcriptomic microarray dataset. The dataset comprises human skeletal muscle samples, obtained from healthy males and females, that were used to derive a gene signature of a high informative content, with respect to its functional association with the aging phenotype. Towards this end, a multilayered computational workflow integrating advanced statistical methodologies for the derivation of reliable confidence measures, distribution-based entropy calculations to examine the informational content of the dataset, enrichment analysis, graph-theoretic methods and intuitive visualization was applied. Specifically, statistical testing revealed differentially expressed genes, while an uncertainty calculation algorithm, exploiting Gene Ontology (GO) terms annotations, extended the list of significant genes from 254 to 2791, namely p-value threshold was increased from 0.0005 to 0.103, while keeping simultaneously noise measurements legitimately low. This rich gene set associated functionally the macroscopic phenotype of muscular aging with highly informative, stably correlated with each other, molecular annotations in the GO database. Finally, a set of 57 reliable genes was identified that comprise a gender-independent aging signature, after incorporating crucial information about genes pivotal regulatory role as inferred by the GO tree. The biological interpretation was highly assisted by the illustration of the functional mappings between genes, cellular location and biological processes through circle packing graphs.
Όνομα εκδήλωσης:	8th International Conference on Systems Biology, ISB 2014
Ημ/νία έναρξης εκδήλωσης :	2014-08-24
Ημ/νία λήξης εκδήλωσης :	2014-08-27
Τίτλος πηγής δημοσίευσης:	International Conference on Systems Biology, ISB
Σελίδες:	156-163
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	aging enrichment analysis entropy functional annotation gene ontology genes muscle visualization
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2014 IEEE.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο