Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Diverse functions of mRNA metabolism factors in stress defense and aging of Caenorhabditis elegans
Δημιουργός/Συγγραφέας:	Rousakis A. Vlanti A. Borbolis F. Roumelioti F. Kapetanou M. Syntichaki P.
Εκδότης:	Public Library of Science
Ημερομηνία:	2014
Γλώσσα:	Αγγλικά
ISSN:	1932-6203
DOI:	10.1371/journal.pone.0103365
Άλλο:	PubMed ID: 25061667
Περίληψη:	Processing bodies (PBs) and stress granules (SGs) are related, cytoplasmic RNA-protein complexes that contribute to post-transcriptional gene regulation in all eukaryotic cells. Both structures contain translationally repressed mRNAs and several proteins involved in silencing, stabilization or degradation of mRNAs, especially under environmental stress. Here, we monitored the dynamic formation of PBs and SGs, in somatic cells of adult worms, using fluorescently tagged protein markers of each complex. Both complexes were accumulated in response to various stress conditions, but distinct modes of SG formation were induced, depending on the insult. We also observed an age-dependent accumulation of PBs but not of SGs. We further showed that direct alterations in PB-related genes can influence aging and normal stress responses, beyond their developmental role. In addition, disruption of SG-related genes had diverse effects on development, fertility, lifespan and stress resistance of worms. Our work therefore underlines the important roles of mRNA metabolism factors in several vital cellular processes and provides insight into their diverse functions in a multicellular organism.
Τίτλος πηγής δημοσίευσης:	PLoS ONE
Τόμος/Κεφάλαιο:	9
Τεύχος:	7
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2014 Rousakis et al.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Gold, Green
Σημειώσεις:	National Institutes of Health, NIH: P40 OD010440; University of Minnesota, UMN; Seventh Framework Programme, FP7: 201975; Ministry of Education, Culture, Sports, Science and Technology, Monbusho.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο