Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Tumor heterogeneity revealed by KRAS, BRAF, and PIK3CA pyrosequencing: KRAS and PIK3CA intratumor mutation profile differences and their therapeutic implications
Δημιουργός/Συγγραφέας:	[EL] Κοσμίδου, Παρασκευή[EN] Kosmidou, Paraskevi Oikonomou E. Vlassi M. Avlonitis S. Katseli A. Tsipras I. Mourtzoukou D. Kontogeorgos G. Zografos G. [EL] Πίντζας, Αλέξανδρος[EN] Pintzas, Alexander
Εκδότης:	John Wiley and Sons Inc
Ημερομηνία:	2014
Γλώσσα:	Αγγλικά
ISSN:	1059-7794
DOI:	10.1002/humu.22496
Άλλο:	PubMed ID: 24352906
Περίληψη:	Current clinical problems in colorectal cancer (CRC) diagnostics and therapeutics include the disease complexity, tumor heterogeneity, and resistance to targeted therapeutics. In the present study, we examined 171 CRC adenocarcinomas from Greek patients undergoing surgery for CRC to determine the frequency of KRAS, BRAF, and PIK3CA point mutations from different areas of tumors in heterogeneous specimens. Ninety two out of 171 (53.8%) patients were found to bear a KRAS mutation in codons 12/13. Of the 126 mutations found, 57.9% (73/126) were c.38G>A mutations (p.G13D) and 22.2% (28/126) were c.35G>T (p.G12V). Remarkably, RAS mutations in both codons 12 and 13 were recorded in the same tumor by pyrosequencing. Moreover, differences in KRAS mutations between tumor center and periphery revealed tumor heterogeneity in 50.7% of the specimens. BRAF c.1799T>A (V600E) mutations were moderately detected in 4/171 (2.3%) specimens, whereas most PIK3CA mutations were revealed by pyrosequencing 6/171 (3.5%). Remarkable tumor heterogeneity is revealed, where double mutations of KRAS in the same tumor and different KRAS mutation status between tumor core and margin are detected with high frequency. It is expected that these findings will have a major impact in cancer diagnosis and personalized therapies.
Τίτλος πηγής δημοσίευσης:	Human Mutation
Τόμος/Κεφάλαιο:	35
Τεύχος:	3
Σελίδες:	329-340
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	BRAF Colorectal cancer KRAS PIK3CA Tumor heterogeneity
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2013 Wiley Periodicals, Inc.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο