Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	HuR-regulated mRNAs associated with nuclear hnRNP A1-RNP complexes
Δημιουργός/Συγγραφέας:	[EL] Παπαδόδημα, Όλγα[EN] Papadodima, Olga [EL] Χατζηιωάννου, Αριστοτέλης[EN] Chatziioannou, Aristotelis [EL] Πατρινού-Γεωργούλα, Μερόπη[EN] Patrinou-Georgoula, Meropi [EL] Κολίσης, Φραγκίσκος Ν.[EN] Kolisis, Fragiskos N.‏ [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki [EL] Γκιάλη, Αποστολία[EN] Guialis, Apostolia
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	1661-6596
DOI:	10.3390/ijms141020256
Άλλο:	PubMed ID: 24152440
Περίληψη:	Post-transcriptional regulatory networks are dependent on the interplay of many RNA-binding proteins having a major role in mRNA processing events in mammals. We have been interested in the concerted action of the two RNA-binding proteins hnRNP A1 and HuR, both stable components of immunoselected hnRNP complexes and having a major nuclear localization. Specifically, we present here the application of the RNA-immunoprecipitation (RIP)-Chip technology to identify a population of nuclear transcripts associated with hnRNP A1-RNPs as isolated from the nuclear extract of either HuR WT or HuR-depleted (KO) mouse embryonic fibroblast (MEF) cells. The outcome of this analysis was a list of target genes regulated via HuR for their association (either increased or reduced) with the nuclear hnRNP A1-RNP complexes. Real time PCR analysis was applied to validate a selected number of nuclear mRNA transcripts, as well as to identify pre-spliced transcripts (in addition to their mature mRNA counterpart) within the isolated nuclear hnRNP A1-RNPs. The differentially enriched mRNAs were found to belong to GO categories relevant to biological processes anticipated for hnRNP A1 and HuR (such as transport, transcription, translation, apoptosis and cell cycle) indicating their concerted function in mRNA metabolism.
Τίτλος πηγής δημοσίευσης:	International Journal of Molecular Sciences
Τόμος/Κεφάλαιο:	14
Τεύχος:	10
Σελίδες:	20256-20281
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	Mouse embryonic fibroblasts (MEFs) MRNA processing Post-transcriptional regulation Ribonucleoprotein (hnRNP) complexes RNA-binding proteins (RBPs) RNA-immunoprecipitation (RIP)-Chip technology
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2013 by the authors; licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Gold, Green
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο