Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Development of amphiphilic block copolymers as silica optical fiber overlayers for BSA protein detection
Δημιουργός/Συγγραφέας:	Petropoulou A. Gibson T.J. Themistou E. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios [EL] Ριζιώτης, Χρήστος Δ.[EN] Riziotis, Christos
Εκδότης:	Elsevier Ltd
Ημερομηνία:	2018
Γλώσσα:	Αγγλικά
ISSN:	0254-0584
DOI:	10.1016/j.matchemphys.2018.06.027
Περίληψη:	Novel amphiphilic block copolymers having both hydrophobic poly(methyl methacrylate) (PMMA) and hydrophilic poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) blocks have been designed and synthesized for efficient protein detection in photonic-based sensing. Both the cationic PMMA117-b-PDMAEMA16 and the cationic vinyl-sulfone functionalized PMMA117-b-P(DMAEMA17-VSTEMA2) block copolymers, where VSTEMA is 2-(2-(vinylsulfonyl)ethylthio)ethyl methacrylate, were synthesized from a water insoluble hydrophobic PMMA block, which facilitated the formation of stable overlayers on the silica optical fibers surface. The well-defined structure of the co-polymers was confirmed by gel permeation chromatography (GPC). The presence of the cationic PDMAEMA block and the vinyl-sulfone double bonds led to reversible electrostatic binding of negatively charged proteins like bovine serum albumin (BSA) and non-reversible chemical binding by thiol-ene reactions with cysteine in proteins, respectively. The sensing properties of these materials were assessed and confirmed by ATR-FTIR analysis and by the characterization of fabricated sensing heads on silica optical fibers functionalized with suitably deposited overlayers. The sensing assessment revealed the requirements for deposited overlayer characteristics towards proteins' detection sensitivity and selectivity enhancement.
Τίτλος πηγής δημοσίευσης:	Materials Chemistry and Physics
Τόμος/Κεφάλαιο:	216
Σελίδες:	421-428
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Λέξεις-Κλειδιά:	Biosensing Block copolymers Optical fibers Photonic sensors Polymers Proteins
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2018 Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Green
Σημειώσεις:	European Commission, EC; European Regional Development Fund, FEDER We acknowledge support of this work by the project “ Advanced Materials and Devices ” ( MIS 5002409 ) which is implemented under the “Action for the Strategic Development on the Research and Technological Sector”, funded by the Operational Programme “ Competitiveness, Entrepreneurship and Innovation ” (NSRF 2014–2020) and co-financed by Greece and the European Union (European Regional Development Fund ). The COST Action BM1401 “ Raman-based Applications for Clinical Diagnostics (Raman4Clinics) ” is also acknloweledged.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο