Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes
Δημιουργός/Συγγραφέας:	Vasilatou D. Papageorgiou S.G. Kontsioti F. Kontos C.K. Tsiotra P. Mpakou V. Pavlou M.A.S. Economopoulou C. [EL] Δημητριάδης, Γιώργος[EN] Dimitriadis, Giorgos Dervenoulas J. Pappa V.
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	0145-2126
DOI:	10.1016/j.leukres.2012.11.011
Άλλο:	PubMed ID: 23246221
Περίληψη:	Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients.
Τίτλος πηγής δημοσίευσης:	Leukemia Research
Τόμος/Κεφάλαιο:	37
Τεύχος:	3
Σελίδες:	251-258
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Γενετική[EN] Genetics
Λέξεις-Κλειδιά:	Let-7a MDS MicroRNAs Mir-17-5p Mir-20a
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2012 Elsevier Ltd.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο