Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues
Δημιουργός/Συγγραφέας:	Georgakopoulou E.A. Tsimaratou K. Evangelou K. Fernandez-Marcos P.J. [EL] Ζουμπουρλής, Βασίλης[EN] Zoumpourlis, Vassilis [EL] Τρουγκάκος, Ιωάννης Π.[EN] Trougakos, Ioannis P. Kletsas D. Bartek J. Serrano M. Gorgoulis V.G.
Εκδότης:	Impact Journals LLC
Ημερομηνία:	2013
Γλώσσα:	Αγγλικά
ISSN:	1945-4589
DOI:	10.18632/aging.100527
Περίληψη:	There is shortage of extensive clinicopathologic studies of cellular senescence because the most reliable senescence biomarker, the detection of Senescence-Associated-beta-galactosidase activity (SA-ß-gal), is inapplicable in archival material and requires snap-frozen tissues. We validated the histochemical Sudan-Black-B (SBB) specific stain of lipofuscin, an aggregate of oxidized proteins, lipids and metals, known to accumulate in aged tissues, as an additional reliableapproach to detect senescent cells independently of sample preparation. We analyzed cellular systems in which senescencewas triggered by replicative exhaustion or stressful stimuli, conditional knock-in mice producing precancerous lesions exhibiting senescence, and human preneoplastic lesions known to contain senescent cells. In the above settings we demonstrated co-localization of lipofuscin and SA-ß-gal in senescent cells in vitro and in vivo (cryo-preserved tissue), strongly supporting the candidacy of lipofuscin for a biomarker of cellular senescence. Furthermore, cryo-preserved tissues positive for SA-ß-gal were formalin-fixed, paraffin-embedded, and stained with SBB. The corresponding SA-ß-gal positive tissue areas stained specifically for lipofuscin by SBB, whereas tissues negative for SA-ß-gal were lipofuscin negative, validating the sensitivity and specificity of the SBB staining to visualize senescent cells in archival material. The latter unique property of SBB could be exploited in research on widely available retrospective tissue material.
Τίτλος πηγής δημοσίευσης:	Aging
Τόμος/Κεφάλαιο:	5
Τεύχος:	1
Σελίδες:	37-50
Θεματική Κατηγορία:	[EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	Biomarker Cellular senescence Formalin-fixed paraffin-embedded Lipofuscin Sudan Black B
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© Georgakopoulou et al.
Όροι και προϋποθέσεις δικαιωμάτων:	All Open Access, Gold, Green
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο