Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Adsorption of lysozyme on pH-responsive PnBA-b-PAA polymeric nanoparticles: studies by stopped-flow SAXS and ITC
Δημιουργός/Συγγραφέας:	Filippov S.K. [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis Riabtseva A. [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Εκδότης:	Springer Verlag
Ημερομηνία:	2018
Γλώσσα:	Αγγλικά
ISSN:	0303-402X
DOI:	10.1007/s00396-018-4329-4
Περίληψη:	The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA block copolymer had a spherical shape and core-shell structure, which was confirmed by cryo-transmission electron microscopy (cryo-TEM) and SAXS. ITC revealed that lysozyme binds to the nanoparticle surface and that the strength of the binding depends on the ionic strength of the solution and the composition of the PnBA-b-PAA copolymers. Thermodynamic results unambiguously indicated that the interaction of lysozyme with the nanoparticle surface is a two-step process and entropy driven. Stopped-flow experiments proved that lysozyme penetration into the PAA corona occurs on a time scale of hundreds of milliseconds. Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.
Τίτλος πηγής δημοσίευσης:	Colloid and Polymer Science
Τόμος/Κεφάλαιο:	296
Τεύχος:	7
Σελίδες:	1183-1191
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Λέξεις-Κλειδιά:	Cryo-TEM Isothermal titration calorimetry Lysozyme Polymeric nanoparticles Small-angle X-ray scattering Stopped-flow
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Σημειώσεις:	Ministerstvo Školství, Mládeže a Tělovýchovy, MŠMT: LH15213 Funding information We acknowledge financial support from the Ministry of Education, Youth and Sports of the Czech Republic, grant no. LH15213.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο